Effect of mercury on the hepato-nephrocitic system of Bombus atratus Franklin (Hymenoptera: Bombini)

• Grant number: 17/06056-9
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2017
• End date: September 30, 2019
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Fábio Camargo Abdalla
• Grantee: Fábio Camargo Abdalla
• Host Institution: Centro de Ciências Humanas e Biológicas (CCHB). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil

Abstract

Most studies related to the disappearance and decreased of Bombus regards population survey and mortality in a particular region or locality. There is not significative studies about the impact of trace metals on the morphology of internal organs of neotropical native bees, despite such toxicants are present in most industrial waste released in air, soil and water. Therefore, the project aims to improve the knowledge of the impact of trace metals, such as mercury (Hg), in important internal organs of these bees. The study will analyze, in a comparative fashion and in replicate, the hepato-nephrocitic system of workers of B. atratus collected in the field and kept under controlled conditions of temperature and humidity bioassays. Experimental groups (n = 10 for each experiment), will receive natural food ad libitum (mixture of pollen, nectar and organic soy flour) and daily 2 mL of solution containing 0.2 ppb Hg (maximum concentration of mercury considered safe for Class II and Class VII waters, CONAMA) and 110 ppb (approximate average value found in honey pots in native stingless bee from Brazil, Argentina, Venezuela and Australia. - Pablo et al, 2012). For the control group (n = 10) will be offered food and 2 mL of mineral water. After exposure of 48h, the bees under crio-anesthesia at 4 oc will sacrificed and their internal organs (dorsal vessel, pericardial cells, fat body and hemocytes) will processed for routine, histochemical and immune-histochemical analyses. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BALSAMO, PAULO JOSE; DA COSTA DOMINGUES, CAIO EDUARDO; DA SILVA-ZACARIN, ELAINE CRISTINA MATHIAS; GREGORC, ALES; IRAZUSTA, SILVIA PIERRE; SALLA, RAQUEL FERNANDA; COSTA, MONICA JONES; ABDALLA, FABIO CAMARGO. Impact of sublethal doses of thiamethoxam and Nosema ceranae inoculation on the hepato-nephrocitic system in young Africanized Apis mellifera. JOURNAL OF APICULTURAL RESEARCH, v. 59, n. 4, NOV 2019. (14/04697-9, 17/06056-9, 11/17840-6, 13/09419-4)