Molecular epidemiology of Leptospira noguchii reveals important insights into a One Health context

Autor(es)

Loureiro, Ana P. ^[1] ; Jaeger, Lauren H. ^{[1, 2]} ; Di Azevedo, Maria Isabel N. ^[1] ; Miraglia, Fabiana ^{[3, 1]} ; Moreno, Luisa Z. ^[3] ; Moreno, Andrea M. ^[3] ; Pestana, Cristiane P. ^[4] ; Carvalho-Costa, Filipe A. ^[2] ; Medeiros, Marco A. ^[4] ; Lilenbaum, Walter ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Fluminense, Dept Microbiol & Parasitol, Lab Bacteriol Vet, Niteroi, RJ - Brazil

^[2] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Epidemiol & Sistemat Mol, Rio De Janeiro - Brazil

^[3] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo - Brazil

^[4] Fiocruz MS, Lab Tecnol Recombinante Biomanguinh, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TRANSBOUNDARY AND EMERGING DISEASES; v. 67, n. 1 SEP 2019.
• Citações Web of Science: 0

Resumo

Leptospirosis presents a complex and dynamic epidemiology. Bovine leptospirosis has been described as a major infectious disease impairing reproductive efficiency. Although infections by Leptospira interrogans, L. santarosai and L. borgpetersenii are frequently reported in cattle, the presence of L. noguchii in these animals should not be neglected. In this study, we describe serological (MAT) and molecular characterization (rrs and secY gene sequencing, multilocus sequence typing {[}MLST] and pulsed-field gel electrophoresis {[}PFGE]) of eight L. noguchii strains obtained from slaughtered cows. Intraspecific genetic diversity was evaluated, and haplotype networks were constructed based on hosts and geographical localizations. Strains were characterized as belonging to serogroups Australis, Autumnalis and Panama, and molecular characterization showed a high heterogeneity of these strains. Ten different STs were found (including nine new STs and 39 novel alleles) as well as nine different pulsotypes. Two clonal complexes were found. Phylogenetic trees based on secY locus and concatenated MLST loci showed two main clusters, with sequences from the present study included in the first. In general, there was no relationship between the geographical origin and the secY phylogenetic clusters, as well as between secY phylogenetic clusters and serogroups. Molecular diversity indexes confirmed a high variability (H > 0.8). This high intraspecific variation observed may be related to differences in virulence, pathogenicity and antigenicity or even adaptability of the strains. In addition, haplotype networks clearly demonstrated the circulation of genotypes between humans and animals, confirming the zoonotic potential. The present study provides relevant data for the study of leptospirosis in the One Health context, where human, animal and environmental health is closely connected. (AU)

• Processo FAPESP: 13/17136-2 - Caracterização de estirpes de Leptospira isoladas no Brasil através de tipificação por eletroforese em campo pulsado (PFGE) e análise comparativa de genomas.
• Beneficiário: Luisa Zanolli Moreno
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/18290-0 - Caracterização de estirpes de Leptospira isoladas no Brasil através da tipagem por sequenciamento de multilocus (MLST)
• Beneficiário: Andrea Micke Moreno
• Modalidade de apoio: Auxílio à Pesquisa - Regular