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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular epidemiology of Leptospira noguchii reveals important insights into a One Health context

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Author(s):
Loureiro, Ana P. [1] ; Jaeger, Lauren H. [1, 2] ; Di Azevedo, Maria Isabel N. [1] ; Miraglia, Fabiana [3, 1] ; Moreno, Luisa Z. [3] ; Moreno, Andrea M. [3] ; Pestana, Cristiane P. [4] ; Carvalho-Costa, Filipe A. [2] ; Medeiros, Marco A. [4] ; Lilenbaum, Walter [1]
Total Authors: 10
Affiliation:
[1] Univ Fed Fluminense, Dept Microbiol & Parasitol, Lab Bacteriol Vet, Niteroi, RJ - Brazil
[2] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Epidemiol & Sistemat Mol, Rio De Janeiro - Brazil
[3] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo - Brazil
[4] Fiocruz MS, Lab Tecnol Recombinante Biomanguinh, Rio De Janeiro - Brazil
Total Affiliations: 4
Document type: Journal article
Source: TRANSBOUNDARY AND EMERGING DISEASES; v. 67, n. 1 SEP 2019.
Web of Science Citations: 0
Abstract

Leptospirosis presents a complex and dynamic epidemiology. Bovine leptospirosis has been described as a major infectious disease impairing reproductive efficiency. Although infections by Leptospira interrogans, L. santarosai and L. borgpetersenii are frequently reported in cattle, the presence of L. noguchii in these animals should not be neglected. In this study, we describe serological (MAT) and molecular characterization (rrs and secY gene sequencing, multilocus sequence typing {[}MLST] and pulsed-field gel electrophoresis {[}PFGE]) of eight L. noguchii strains obtained from slaughtered cows. Intraspecific genetic diversity was evaluated, and haplotype networks were constructed based on hosts and geographical localizations. Strains were characterized as belonging to serogroups Australis, Autumnalis and Panama, and molecular characterization showed a high heterogeneity of these strains. Ten different STs were found (including nine new STs and 39 novel alleles) as well as nine different pulsotypes. Two clonal complexes were found. Phylogenetic trees based on secY locus and concatenated MLST loci showed two main clusters, with sequences from the present study included in the first. In general, there was no relationship between the geographical origin and the secY phylogenetic clusters, as well as between secY phylogenetic clusters and serogroups. Molecular diversity indexes confirmed a high variability (H > 0.8). This high intraspecific variation observed may be related to differences in virulence, pathogenicity and antigenicity or even adaptability of the strains. In addition, haplotype networks clearly demonstrated the circulation of genotypes between humans and animals, confirming the zoonotic potential. The present study provides relevant data for the study of leptospirosis in the One Health context, where human, animal and environmental health is closely connected. (AU)

FAPESP's process: 13/17136-2 - Characterization of Leptospira strains isolated in Brazil by pulsed field gel electrophoresis (PFGE) typing and comparative genomic analysis.
Grantee:Luisa Zanolli Moreno
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/18290-0 - Characterization of Leptospira strains isolated in Brazil through multiloccus sequence typing (MLST)
Grantee:Andrea Micke Moreno
Support Opportunities: Regular Research Grants