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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

New Disposable Electrochemical Paper-based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample

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Autor(es):
Fava, Elson Luiz [1] ; do Prado, Thiago Martimiano [1] ; Silva, Tiago Almeida [1, 2] ; de Moraes, Fernando Cruz [1] ; Faria, Ronaldo Censi [1] ; Fatibello-Filho, Orlando [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Dept Chem, POB 676, BR-13560970 Sao Carlos, SP - Brazil
[2] Fed Ctr Technol Educ Minas Gerais, Dept Met & Chem, BR-35180008 Timoteo, MG - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Electroanalysis; v. 32, n. 5 JAN 2020.
Citações Web of Science: 0
Resumo

A disposable electrochemical paper-based analytical device was constructed based on use of sequential analysis with multiplexed working electrodes and applied for the determination of glucose, creatinine, and uric acid. The device was constructed with 16 microfluidic channels, with 16 working electrodes arranged in four set with four components surrounding the sample injection hole. In addition, a commercial multiplexing module was used, which allowed for multiplexing of the 16 working electrodes. This design allowed for radial and homogeneous sample elution to each sensing spot for high throughput analysis. In the multiplexed determinations, distinct electrochemical procedures were employed for each analyte. Furthermore, each working electrode spot was modified to increase the respective analytical signals. For glucose detection, the sensor was based on electron mediation by ferrocenecarboxylic acid over the modified surface with glucose oxidase. The principle for creatinine detection was based on electrochemical reduction of non-complexed Fe3+ in excess after complex formation between Fe3+ and creatinine in the chemical step. The anodic peak current responses for uric acid detection increased due to working electrode surface modification with carbon black nanoparticles. In the multiplexed analysis, the device provided limits of detection of 0.120 mmol L-1, 0.084 mmol L-1, and 0.012 mmol L-1 for glucose, creatinine, and uric acid, respectively. The developed device was successfully applied in the analyses of real urine samples. (AU)

Processo FAPESP: 17/24053-7 - Desenvolvimentos de métodos eletroquímicos simples para a detecção de biomarcadores visando o diagnóstico de doenças
Beneficiário:Ronaldo Censi Faria
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 16/10012-4 - Desenvolvimento de dispositivo microfluídico descartável contendo arranjos de biossensores para a determinação multiplexada de espécies de interesse biológico em diferentes matrizes
Beneficiário:Elson Luiz Fava
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 17/10118-0 - Estudo e aplicação da tecnologia eletroquímica para a análise e a degradação de interferentes endócrinos: materiais, sensores, processos e divulgação científica
Beneficiário:Marcos Roberto de Vasconcelos Lanza
Linha de fomento: Auxílio à Pesquisa - Temático