New Disposable Electrochemical Paper-based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample

Author(s)

Fava, Elson Luiz ^[1] ; do Prado, Thiago Martimiano ^[1] ; Silva, Tiago Almeida ^{[1, 2]} ; de Moraes, Fernando Cruz ^[1] ; Faria, Ronaldo Censi ^[1] ; Fatibello-Filho, Orlando ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Chem, POB 676, BR-13560970 Sao Carlos, SP - Brazil

^[2] Fed Ctr Technol Educ Minas Gerais, Dept Met & Chem, BR-35180008 Timoteo, MG - Brazil

• Document type: Journal article
• Source: Electroanalysis; v. 32, n. 5 JAN 2020.
• Web of Science Citations: 0

Abstract

A disposable electrochemical paper-based analytical device was constructed based on use of sequential analysis with multiplexed working electrodes and applied for the determination of glucose, creatinine, and uric acid. The device was constructed with 16 microfluidic channels, with 16 working electrodes arranged in four set with four components surrounding the sample injection hole. In addition, a commercial multiplexing module was used, which allowed for multiplexing of the 16 working electrodes. This design allowed for radial and homogeneous sample elution to each sensing spot for high throughput analysis. In the multiplexed determinations, distinct electrochemical procedures were employed for each analyte. Furthermore, each working electrode spot was modified to increase the respective analytical signals. For glucose detection, the sensor was based on electron mediation by ferrocenecarboxylic acid over the modified surface with glucose oxidase. The principle for creatinine detection was based on electrochemical reduction of non-complexed Fe3+ in excess after complex formation between Fe3+ and creatinine in the chemical step. The anodic peak current responses for uric acid detection increased due to working electrode surface modification with carbon black nanoparticles. In the multiplexed analysis, the device provided limits of detection of 0.120 mmol L-1, 0.084 mmol L-1, and 0.012 mmol L-1 for glucose, creatinine, and uric acid, respectively. The developed device was successfully applied in the analyses of real urine samples. (AU)

• FAPESP's process: 17/24053-7 - Development of simple electrochemical methods for the detection of biomarkers for the diagnosis of diseases
• Grantee: Ronaldo Censi Faria
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/10012-4 - Development of a disposable biosensors array with a microfluidic device for multiplex determination of species of biological interest in different matrices
• Grantee: Elson Luiz Fava
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/10118-0 - Study and application of electrochemical technology for the analysis and degradation of endocrine interferents: materials, sensors, processes and scientific dissemination
• Grantee: Marcos Roberto de Vasconcelos Lanza
• Support Opportunities: Research Projects - Thematic Grants