Effects of bromide-enriched natural seawater culture medium on protein and monoterpenes output of Ochtodes secundiramea (Rhodophyta, Gigartinales)

Autor(es)

Machado, Levi P. ^[1] ; Stefanello, Eliezer ^[2] ; Andreghetti, Daniel X. ^[2] ; Zambotti-Villela, Leonardo ^[2] ; Colepicolo, Pio ^[2] ; de Carvalho, Luciana R. ^[3] ; Yokoya, Nair S. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Coordenadoria Engn Pesca, Campus Expt Registro, BR-11900000 Registro, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil

^[3] Secretaria Estado Meio Ambiente, Inst Bot, Nucleo Pesquisa Ficol, Av Miguel Estefano 3687, BR-04301012 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF APPLIED PHYCOLOGY; v. 31, n. 6, p. 3831-3839, DEC 2019.
• Citações Web of Science: 0

Resumo

Benthic marine algae, especially Rhodophyta, are within a well-known marine group able to produce secondary metabolites with bioactive properties. Among them are halogenated terpenes, synthesized by vanadium-dependent haloperoxidase (V-BPO). The red alga Ochtodes secundiramea (Montagne) M. Howe produces high amounts of halogenated monoterpenes (HMT) which have the potential to control phytopathogenic fungi. Culture closed a system is a tool used to manipulate conditions to increase the synthesis of HMT and other metabolites. The apical segments of O. secundiramea were cultivated in a bioreactor, either in seawater enriched with half strength von Stosch's solution (VSES/2) without (control) and with the addition of 250 mg L-1 bromine (treatment). In response to these conditions, specific growth rate, HMT profile, soluble protein, phycobiliproteins, soluble carbohydrate, and chlorophyll as well as the V-BPO activity, uptake, and accumulated bromine within the biomass were evaluated. The treatment did not promote significant alterations in the growth but increased the V-BPO activity and bromine uptake and incorporation (2.5 fold). No changes in the HMT profiles of the extracts from both cultures conditions were observed by gas chromatography-mass spectrometry analyses, whereas the values of total soluble protein and phycobiliproteins were 2 fold higher in samples from the bromine-enriched medium when compared to samples from control. These results support the evidences of protective effects promoted by V-BPO activity. Furthermore, carbohydrate and chlorophyll contents were not affected by experimental conditions. These results provided new low-cost strategies that can be applied to increase of certain biomass components, which here are algal proteins, especially in Rhodophyta-based biotechnological industries for food and agriculture. (AU)

• Processo FAPESP: 10/14893-9 - Estudo dos efeitos metabólicos de antraceno sobre a microalga Chlamydomonas reinhardtii
• Beneficiário: Eliezer Stefanello
• Modalidade de apoio: Bolsas no Brasil - Doutorado