Dose-dependent cell necrosis induced by silica nanoparticles

Autor(es)

Reus, Thamile Luciane ^{[1, 2, 3]} ; Marcon, Bruna Hilzendeger ^[1] ; Campos Paschoal, Ariane Caroline ^[1] ; Sousa Ribeiro, Iris Renata ^{[4, 5, 6]} ; Cardoso, Mateus Borba ^{[4, 5, 6]} ; Dallagiovanna, Bruno ^[1] ; de Aguiar, Alessandra Melo ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] FIOCRUZ Parana, Lab Biol Basica Celulas Tronco, Inst Carlos Chagas, BR-81350010 Curitiba, Parana - Brazil

^[2] Univ Fed Parana, Programa Posgrad Biol Celular & Mol, BR-81531990 Curitiba, Parana - Brazil

^[3] Grp Bot Pesquisa & Desenvolvimento, Ave Rui Barbosa 4110, BR-83055320 Sao Jose Dos Pinhais, Parana - Brazil

^[4] Univ Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP - Brazil

^[5] CNPEM, LNLS, Campinas, SP - Brazil

^[6] CNPEM, Lab Nacl Nanotecnol LNNano, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY IN VITRO; v. 63, MAR 2020.
• Citações Web of Science: 0

Resumo

In recent years, much attention has been given to nanoparticles (NPs) due to their many possible applications, and as research has progressed, these NPs have become valuable tools for medical purposes. Among many different types of NPs, silica nanoparticles (SiO(2)NPs) have been specifically evaluated for medical purposes and have also been used in many different types of products. Although SiO(2)NPs have already been applied and are believed to be nontoxic, there is still a concern regarding possible adverse effects that may be triggered after SiO2NP exposure. Therefore, in the present study, we employed a recommended cell line (BALB/c 3T3) for the toxicity evaluation to investigate the cytotoxic effects of SiO(2)NPs produced by chemical synthesis at a laboratory scale. First, we employed OECD guideline 129 in order to evaluate cytotoxicity effects and also estimate the starting doses for acute oral systemic toxicity tests. We evaluated the cytotoxic effects of two types of SiO(2)NPs (nonfluorescent and fluorescent) and found that they were not significantly different (IC50 = 1986.39 +/- 237 mu g/mL and IC50 = 1861.13 +/- 186.72 mu g/mL, respectively). Then, we used the predicted LD50 of both types of SiO(2)NPs to suggest that they could be categorized as GHS category 4 substances. By ultrastructural evaluation, we found that SiO(2)NPs are internalized by 3 T3 cells and are located in vacuolelike structures with no other significant changes in cell structure. We also found that SiO(2)NPs lead to cell necrosis in a dose-dependent manner. (AU)

• Processo FAPESP: 15/25406-5 - Organizando a matéria: colóides formados por associação de surfactantes, polímeros e nanopartículas
• Beneficiário: Watson Loh
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/21318-0 - Direcionamento de nanopartículas funcionalizadas em sistemas microfluídicos contendo múltiplos organoides
• Beneficiário: Iris Renata Sousa Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado