Dose-dependent cell necrosis induced by silica nanoparticles

Author(s)

Reus, Thamile Luciane ^{[1, 2, 3]} ; Marcon, Bruna Hilzendeger ^[1] ; Campos Paschoal, Ariane Caroline ^[1] ; Sousa Ribeiro, Iris Renata ^{[4, 5, 6]} ; Cardoso, Mateus Borba ^{[4, 5, 6]} ; Dallagiovanna, Bruno ^[1] ; de Aguiar, Alessandra Melo ^{[1, 2]}

Affiliation

^[1] FIOCRUZ Parana, Lab Biol Basica Celulas Tronco, Inst Carlos Chagas, BR-81350010 Curitiba, Parana - Brazil

^[2] Univ Fed Parana, Programa Posgrad Biol Celular & Mol, BR-81531990 Curitiba, Parana - Brazil

^[3] Grp Bot Pesquisa & Desenvolvimento, Ave Rui Barbosa 4110, BR-83055320 Sao Jose Dos Pinhais, Parana - Brazil

^[4] Univ Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP - Brazil

^[5] CNPEM, LNLS, Campinas, SP - Brazil

^[6] CNPEM, Lab Nacl Nanotecnol LNNano, Campinas, SP - Brazil

• Document type: Journal article
• Source: TOXICOLOGY IN VITRO; v. 63, MAR 2020.
• Web of Science Citations: 0

Abstract

In recent years, much attention has been given to nanoparticles (NPs) due to their many possible applications, and as research has progressed, these NPs have become valuable tools for medical purposes. Among many different types of NPs, silica nanoparticles (SiO(2)NPs) have been specifically evaluated for medical purposes and have also been used in many different types of products. Although SiO(2)NPs have already been applied and are believed to be nontoxic, there is still a concern regarding possible adverse effects that may be triggered after SiO2NP exposure. Therefore, in the present study, we employed a recommended cell line (BALB/c 3T3) for the toxicity evaluation to investigate the cytotoxic effects of SiO(2)NPs produced by chemical synthesis at a laboratory scale. First, we employed OECD guideline 129 in order to evaluate cytotoxicity effects and also estimate the starting doses for acute oral systemic toxicity tests. We evaluated the cytotoxic effects of two types of SiO(2)NPs (nonfluorescent and fluorescent) and found that they were not significantly different (IC50 = 1986.39 +/- 237 mu g/mL and IC50 = 1861.13 +/- 186.72 mu g/mL, respectively). Then, we used the predicted LD50 of both types of SiO(2)NPs to suggest that they could be categorized as GHS category 4 substances. By ultrastructural evaluation, we found that SiO(2)NPs are internalized by 3 T3 cells and are located in vacuolelike structures with no other significant changes in cell structure. We also found that SiO(2)NPs lead to cell necrosis in a dose-dependent manner. (AU)

• FAPESP's process: 15/25406-5 - Organizing matter: colloids formed by association of surfactants, polymers and nanoparticles
• Grantee: Watson Loh
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/21318-0 - Targeting of functionalized nanoparticles in microfluidics systems containing multiple organoids
• Grantee: Iris Renata Sousa Ribeiro
• Support Opportunities: Scholarships in Brazil - Doctorate