Evolutionary Dynamics of Multigene Families in Triportheus (Characiformes, Triportheidae): A Transposon Mediated Mechanism?

Autor(es)

Yano, Cassia F. ^[1] ; Merlo, Manuel A. ^[2] ; Portela-Bens, Silvia ^[2] ; Cioffi, Marcelo de B. ^[3] ; Bertollo, Luiz A. C. ^[3] ; Santos-Junior, Celio D. ^[3] ; Rebordinos, Laureana ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Mato Grosso, Dept Biol & Zool, Cuiaba - Brazil

^[2] Univ Cadiz, Dept Biomed Biotecnol & Salud Publ, Fac Ciencias Mar & Ambientales, Puerto Real - Spain

^[3] Univ Fed Sao Carlos, Dept Geneit & Evolucao, Sao Carlos - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MARINE SCIENCE; v. 7, JAN 24 2020.
• Citações Web of Science: 0

Resumo

Triportheus (Characiformes, Triportheidae) is a freshwater fish genus with 18 valid species. These fishes are widely distributed in the major river drainages of South America, having commercial importance in the fishing market, mainly in the Amazon basin. This genus has diverged recently in a complex process of speciation carried out in different river basins. The use of repetitive sequences is suitable to trace the genomic reorganizations occured along the speciation process. In this work, the 5S rDNA multigene family has been characterized at molecular and phylogenetic level. The results showed that other multigene family has been found within the non-transcribed spacer (NTS): the U1 snRNA gene. Double-FISH with 5S and U1 probes were also performed, confirming the close linkage between these two multigene families. Moreover, evidences of different transposable elements (TE) were detected within the spacer, thus suggesting a transposon-mediated mechanism of 5S-U1 evolutionary pathway in this genus. Phylogenetic analysis demonstrated a species-specific grouping, except for Triportheus pantanensis, Triportheus aff. rotundatus and Triportheus trifurcatus. The evolutionary model of the 5S rDNA in Triportheus species has been discussed. In addition, the results suggest new clues for the speciation and evolutionary trend in these species, which could be suitable to use in other Characiformes species. (AU)

• Processo FAPESP: 18/22033-1 - Uma abordagem intercontinental para a investigação da evolução cromossômica, diversidade genética e biogeografia na ordem Osteoglossiformes (Teleostei: Osteoglossomorpha). Parte III.
• Beneficiário: Marcelo de Bello Cioffi
• Linha de fomento: Auxílio à Pesquisa - Regular