Novel small IncX3 plasmid carrying the bla(KPC-2) gene in high-risk Klebsiella pneumoniae ST11/CG258

Autor(es)

Fuga, Bruna ^[1] ; Ferreira, Melina Lorraine ^[1] ; Cerdeira, Louise Teixeira ^[2] ; de Campos, Paola Amaral ^[1] ; Dias, Vinicius Lopes ^[1] ; Rossi, Iara ^[1] ; Machado, Luiz Gustavo ^[1] ; Lincopan, Nilton ^[2] ; Gontijo-Filho, Paulo Pinto ^[1] ; Ribas, Rosineide Marques ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Uberlandia, Inst Ciencias Biomed, Lab Microbiol Mol, Uberlandia, MG - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 96, n. 2 FEB 2020.
• Citações Web of Science: 0

Resumo

This study used whole-genome sequencing (WGS) and PFGE to analysis KPC-2-producing Klebsiella pneumoniae strains from clinical specimens collected in Brazilian hospitals. The study identifies the emergence of a novel small IncX3 plasmid (pKPB11), 12,757-bp in length, in a high-risk K. pneumoniae ST11/CG258 lineage, a successful clonal group in Brazil, carrying the bla(KPC)-2 gene on a non similar to Tn4401 genetic element (NTEKPC-Ic). Comparative analysis of the pKPB11 showed that this plasmid reduced its size, losing part of its conjugation apparatus. The pKPB11 was also compared to another strain sequenced in this study (KPC89) that had the hybrid IncX3-IncU plasmid (p1089), of approximately 45 kb in length, similarly carrying the bla(KPC-2) gene on NTEKPC-Ic. To the best of our knowledge, pKPB11 is the first example of small IncX3 plasmid found in a high-risk KPC-2-producing K. pneumoniae ST11/CG258. (C) 2019 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 15/21325-0 - Análise comparativa do resistoma de Klebsiella pneumoniae XDR (NDM-1/KPC-2) pertencente ao complexo clonal (CC) endêmico de alto risco CC258
• Beneficiário: Louise Teixeira Cerdeira
• Modalidade de apoio: Bolsas no Brasil - Doutorado