| Full text | |
| Author(s): |
Fuga, Bruna
[1]
;
Ferreira, Melina Lorraine
[1]
;
Cerdeira, Louise Teixeira
[2]
;
de Campos, Paola Amaral
[1]
;
Dias, Vinicius Lopes
[1]
;
Rossi, Iara
[1]
;
Machado, Luiz Gustavo
[1]
;
Lincopan, Nilton
[2]
;
Gontijo-Filho, Paulo Pinto
[1]
;
Ribas, Rosineide Marques
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Fed Uberlandia, Inst Ciencias Biomed, Lab Microbiol Mol, Uberlandia, MG - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 96, n. 2 FEB 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
This study used whole-genome sequencing (WGS) and PFGE to analysis KPC-2-producing Klebsiella pneumoniae strains from clinical specimens collected in Brazilian hospitals. The study identifies the emergence of a novel small IncX3 plasmid (pKPB11), 12,757-bp in length, in a high-risk K. pneumoniae ST11/CG258 lineage, a successful clonal group in Brazil, carrying the bla(KPC)-2 gene on a non similar to Tn4401 genetic element (NTEKPC-Ic). Comparative analysis of the pKPB11 showed that this plasmid reduced its size, losing part of its conjugation apparatus. The pKPB11 was also compared to another strain sequenced in this study (KPC89) that had the hybrid IncX3-IncU plasmid (p1089), of approximately 45 kb in length, similarly carrying the bla(KPC-2) gene on NTEKPC-Ic. To the best of our knowledge, pKPB11 is the first example of small IncX3 plasmid found in a high-risk KPC-2-producing K. pneumoniae ST11/CG258. (C) 2019 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 15/21325-0 - Comparative analysis of the resistome of Klebsiella pneumoniae XDR (NDM-1/KPC-2) belonging to the endemic high-risk clonal complex CC258 |
| Grantee: | Louise Teixeira Cerdeira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |