Differential expression of inflammatory and anti-inflammatory mediators by M1 and M2 macrophages after photobiomodulation with red or infrared lasers

Autor(es)

Sousa, Kaline de Brito ^[1] ; Setubal Destro Rodrigues, Maria Fernanda ^[1] ; Santos, Debora de Souza ^[1] ; Mesquita-Ferrari, Raquel Agnelli ^{[1, 2]} ; Nunes, Fabio Daumas ^[3] ; Teixeira da Silva, Daniela de Fatima ^[1] ; Bussadori, Sandra Kalil ^{[1, 2]} ; Santos Fernandes, Kristianne Porta ^[1]

Afiliação do(s) autor(es)

^[1] Nove de Julho Univ UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, 249 Vergueiro St, BR-01504001 Sao Paulo, SP - Brazil

^[2] Nove de Julho Univ UNINOVE, Postgrad Program Rehabil Sci, 249 Vergueiro St, BR-01504001 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Oral Pathol, Dent Sch, 2227 Prof Lineu Prestes Ave, Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 35, n. 2, p. 337-343, MAR 2020.
• Citações Web of Science: 0

Resumo

In response to stimuli in the microenvironment, macrophages adopt either the M1 or M2 phenotype to coordinate the tissue repair process. Photobiomodulation (PBM) plays an important role in the modulation of acute inflammation, including cellular influx, macrophage polarization, and the release of inflammatory mediators. The aim of the present study was to evaluate the effects of red and infrared PBM on the mRNA expression of cytokines and chemokines in macrophages polarized to the M1 and M2 phenotypes. J774 macrophages activated to induce M1 (lipopolysaccharide + interferon gamma) or M2 (interleukin-4) phenotypes were irradiated with red or infrared PBM (1 J). After 4 and 24 h, gene expression was analyzed by qPCR. PBM at 660 nm decreased the mRNA expression of CCL3, CXCL2, and TNF-alpha in M1 macrophages and CXCL2 in M2 macrophages 4 h after irradiation. Similarly, PBM at 780 nm decreased mRNA expression levels of CCL3 and IL-6 by M1 macrophages 24 h after irradiation. Moreover, PBM at 780 nm increased the mRNA expression of TGF beta 1 4 h after irradiation and decreased the expression of this gene after 24 h in M2 macrophages. Although red and infrared PBM were able to modulate and reduce M1/M2a-related markers, infrared laser irradiation promoted a temporal increase in the expression of TGF beta 1 in M2 macrophages. Thus, depending on the time PBM is used on injured tissue, different parameters can promote optimal results by modulating specific macrophage phenotypes. (AU)

• Processo FAPESP: 15/21219-6 - Efeito do laser em baixa intensidade sobre a expressão gênica e proteica de diferentes fenótipos de macrófagos
• Beneficiário: Kaline de Brito Sousa
• Modalidade de apoio: Bolsas no Brasil - Mestrado