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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

In Vitro Toxic Effect of Biomaterials Coated with Silver Tungstate or Silver Molybdate Microcrystals

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Autor(es):
Pellissari, Claudia Viviane Guimaraes [1] ; Vergani, Carlos Eduardo [1] ; Longo, Elson [2] ; Pavarina, Ana Claudia [1] ; Sanita, Paula Volpato [1] ; Siqueira, Walter Luiz [3] ; Jorge, Janaina Habib [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Araraquara Dent Sch, Araraquara, SP - Brazil
[2] Fed Univ Sao Carlos UFSCar, Sao Carlos - Brazil
[3] Univ Saskatchewan, Saskatoon, SK - Canada
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Nanomaterials; v. 2020, JAN 28 2020.
Citações Web of Science: 0
Resumo

Purpose. This study evaluated the cytotoxicity of antimicrobial silver tungstate (Ag2WO4) or silver molybdate (Ag2MoO4) microcrystals coating biomaterials. Materials and Methods. The coating procedure was performed onto titanium, zirconia, and acrylic resin specimens. Eluates of the coated specimens were obtained, which were used for cytotoxicity analyses, including Alamar Blue, MTT, and CytoTox-ONE tests. Data were analyzed using two-way ANOVA, followed by the Tukey test (alpha = 0.05). The results of each experimental group were also compared to those of the control of living cells, taken as 100% cell viability. Results. In general, it was observed that the percentage of living cells from all biomaterials coated with both microcrystals was statistically different compared to the ones from the uncoated sample groups, except for the results from MTT of specimens of Ti coated with alpha-Ag2MoO4. All uncoated biomaterials were classified as noncytotoxic by the three assays used in the present study. It was observed that the microcrystals in solution were strongly cytotoxic, with death of almost 100% of cells, from the analysis of the results of the Alamar Blue assay. Conclusion. The most biomaterials coated with both microcrystals showed some degree of cytotoxicity in the different assays. The results described herein should be seen as an alert to the use of microcrystals, which can expose patients to health risks. (AU)

Processo FAPESP: 11/21928-6 - Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme: avaliação do metabolismo celular
Beneficiário:Janaina Habib Jorge
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/01844-8 - Biocompatibilidade de substâncias utilizadas para prevenção ou eliminação de biofilme: avaliação do metabolismo celular.
Beneficiário:Cláudia Viviane Guimarães Pellissari
Linha de fomento: Bolsas no Brasil - Mestrado