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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In Vitro Toxic Effect of Biomaterials Coated with Silver Tungstate or Silver Molybdate Microcrystals

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Pellissari, Claudia Viviane Guimaraes [1] ; Vergani, Carlos Eduardo [1] ; Longo, Elson [2] ; Pavarina, Ana Claudia [1] ; Sanita, Paula Volpato [1] ; Siqueira, Walter Luiz [3] ; Jorge, Janaina Habib [1]
Total Authors: 7
[1] Sao Paulo State Univ UNESP, Araraquara Dent Sch, Araraquara, SP - Brazil
[2] Fed Univ Sao Carlos UFSCar, Sao Carlos - Brazil
[3] Univ Saskatchewan, Saskatoon, SK - Canada
Total Affiliations: 3
Document type: Journal article
Source: Journal of Nanomaterials; v. 2020, JAN 28 2020.
Web of Science Citations: 0

Purpose. This study evaluated the cytotoxicity of antimicrobial silver tungstate (Ag2WO4) or silver molybdate (Ag2MoO4) microcrystals coating biomaterials. Materials and Methods. The coating procedure was performed onto titanium, zirconia, and acrylic resin specimens. Eluates of the coated specimens were obtained, which were used for cytotoxicity analyses, including Alamar Blue, MTT, and CytoTox-ONE tests. Data were analyzed using two-way ANOVA, followed by the Tukey test (alpha = 0.05). The results of each experimental group were also compared to those of the control of living cells, taken as 100% cell viability. Results. In general, it was observed that the percentage of living cells from all biomaterials coated with both microcrystals was statistically different compared to the ones from the uncoated sample groups, except for the results from MTT of specimens of Ti coated with alpha-Ag2MoO4. All uncoated biomaterials were classified as noncytotoxic by the three assays used in the present study. It was observed that the microcrystals in solution were strongly cytotoxic, with death of almost 100% of cells, from the analysis of the results of the Alamar Blue assay. Conclusion. The most biomaterials coated with both microcrystals showed some degree of cytotoxicity in the different assays. The results described herein should be seen as an alert to the use of microcrystals, which can expose patients to health risks. (AU)

FAPESP's process: 11/21928-6 - Biocompatibility of materials used for the prevention or elimination of biofilm: evaluation of cellular metabolism
Grantee:Janaina Habib Jorge
Support type: Regular Research Grants
FAPESP's process: 13/01844-8 - Biocompatibility of substances used for prevention or elimination of biofilm: assessment of cellular metabolism.
Grantee:Cláudia Viviane Guimarães Pellissari
Support type: Scholarships in Brazil - Master