| Grant number: | 13/01844-8 |
| Support Opportunities: | Scholarships in Brazil - Master |
| Start date: | July 01, 2013 |
| End date: | June 30, 2015 |
| Field of knowledge: | Health Sciences - Dentistry - Dental Clinics |
| Principal Investigator: | Janaina Habib Jorge |
| Grantee: | Cláudia Viviane Guimarães Pellissari |
| Host Institution: | Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil |
Abstract In dental prostheses and medical devices, different biomaterials, including ceramics and titanium alloys, may be coated with TiO2 and AgTiO2, in order to improve their biological properties, particularly with regard to adhesion and proliferation of microorganisms. However, the biocompatibilities of different biomaterials coated with nanoparticles have not yet been evaluated and thus studies are needed in order to improve its performance in vivo. Thus, the objective of this study is to assess the cytotoxicity of biomaterials coated with TiO2 nanoparticles and AgTiO2 through different tests, in order to make them viable for clinical use in preventing biofilm. For this shall be assessed four biomaterials: one leucite reinforced ceramics, one alloy of titanium, aluminum and vanadium (Ti-6Al-4V), one polymethylmethacrylate and one silicone addition. For each experimental condition, the test specimens (10 mm in diameter and 1 mm thick) of each biomaterial be prepared and divided into three groups (n = 9): uncoated (control); coated TiO2; coated AgTiO2 . To analyze the effect of cytotoxic substances released by the body-of-evidence will be obtained extracts of water-soluble substances such samples. For this, three bodies of the test piece from each experimental group will be placed into test tubes with 9 ml of DMEM culture medium and incubated for 24 hours. To perform the cytotoxicity assay, 100 ul of the suspension consisted of 1.0 x 105 cells / ml are placed in each compartment of a plate with 96 holes incubated in an incubator with 5% CO2 at 37 ° C for 24 hours. After this incubation period, the culture medium is discarded, remaining adherent cells on the bottom plate and 50 ul of fresh culture medium are placed in each well of the plate along with 50 ul of extract containing the substances released by the bodies of test, which will be incubated for 24 hours. After the period of incubation, cells proliferation are evaluated by testing Alamar Blue cell viability by the level of ATP (adenosine triphosphate) and cell membrane integrity of viable cells. The results of cellular metabolism of viable cells after contact with extracts of the studied materials, shall be submitted to the most appropriate statistical method and the level of 5% significance will be selected (± = 0.05). Moreover, for each test, the results arecompared with the negative control and treatments in different experimental groups are classified according to the cytotoxic effect on scores from 0 to 3. | |
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