Biocompatibility of ±-Ag2WO4, ²-Ag2MoO4 and ±-AgVO3 microcrystals: evaluation of proinflammatory cytokines and matrix metalloproteinases

• Grant number: 18/01677-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2018
• End date: December 31, 2021
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Carlos Eduardo Vergani
• Grantee: Bruna Natalia Alves da Silva Pimentel
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 13/07296-2 - CDMF - Center for the Development of Functional Materials, AP.CEPID

Abstract

Fungal infections are important causes of morbidity and mortality. Currently there are different therapies with antifungal agents used to control these infections; however, the microbial tolerance to these agents has increased. For this reason, our group has evaluated the antifungal activity of silver-containing microcrystals as well as their cytotoxicity on human cells (human gingival fibroblast - FGH - and spontaneously immortalized Normal Oral Keratinocytes - NOK-si). The results obtained to date showed that the microcrystals tested (silver tungstate alpha, silver beta molybdate and alpha silver vanadate) showed fungistatic and fungistatic activity against Candida albicans and, at the same time, these doses showed no cytotoxicity on human cells (FGH and NOK-si). However, there are important gaps in the biocompatibility of microcrystals that should be carefully investigated as the ability to induce inflammatory response and degradation of matrix metalloproteinases (MMPs). The understanding of such responses is fundamental to ensure a better understanding of the mechanisms of inflammatory response and tissue regeneration facing these new materials. Thus, this project aims to evaluate the expression of proinflammatory cytokines (IL-1², IL-6, IL-8 and TNF-±) and MMPs (-8 and -9) in human cell cultures and reconstituted oral mucosa tissue (ROMT) after exposure to inhibitory concentrations and microcrystalline fungicides (±-Ag2WO4, ²-Ag2MoO4 and ±-AgVO3). To evaluate cell viability, the Alamar Blue assays will be performed. qPCR assays (for the evaluation of the gene expression of cytokines and MMPs) will be performed; flow cytometry (for quantification of soluble cytokines); Western Blot (for quantification of soluble MMPs); and zymography (to evaluate the enzymatic activity of MMPs). The results obtained will be submitted to statistical analysis using GraphPad Prism 5 software. A significance level of 5% will be adopted. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, PAULA FABIANA SANTOS; SILVA, ANA CAROLINA ALVES DE PAULA E; DA SILVA PIMENTEL, BRUNA NATALIA ALVES; PINATTI, IVO MATEUS; SIMOES, ALEXANDRE ZIRPOLI; VERGANI, CARLOS EDUARDO; BARRETO-VIEIRA, DEBORA FERREIRA; DA SILVA, MARCOS ALEXANDRE NUNES; MIRANDA, MILENE DIAS; MONTEIRO, MARIA EDUARDA SANTOS; et al. Inactivation of SARS-CoV-2 by a chitosan/alpha-Ag2WO4 composite generated by femtosecond laser irradiation. SCIENTIFIC REPORTS, v. 12, n. 1, p. 18-pg., 2022-05-17. (19/25944-8, 14/14171-4, 19/03722-3, 17/13008-0, 13/07296-2, 18/16578-5, 18/01677-8)

ALVES DA SILVA PIMENTEL, BRUNA NATALIA; DE ANNUNZIO, SARAH RAQUEL; ASSIS, MARCELO; BARBUGLI, PAULA ABOUD; LONGO, ELSON; VERGANI, CARLOS EDUARDO. Biocompatibility and inflammatory response of silver tungstate, silver molybdate, and silver vanadate microcrystals. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 11, p. 13-pg., 2023-07-20. (18/01677-8, 13/07296-2, 22/08487-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PIMENTEL, Bruna Natalia Alves da Silva. Microcristais de tungstato de prata, molibdato de prata e vanadato de prata: biocompatibilidade e atividade antifúngica sobre biofilme de Candida albicans. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.