The Terrestrial Carnivorous Plant Utricularia reniformis Sheds Light on Environmental and Life-Form Genome Plasticity

Autor(es)

Silva, Saura R. ^[1] ; Moraes, Ana Paula ^[2] ; Penha, Helen A. ^[1] ; Juliao, Maria H. M. ^[1] ; Domingues, Douglas S. ^[3] ; Michael, Todd P. ^[4] ; Miranda, Vitor F. O. ^[5] ; Varani, Alessandro M. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Fac Ciencias Agr Vet, Dept Tecnol, BR-14884900 Jaboticabal - Brazil

^[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09606070 Sao Bernardo Do Campo - Brazil

^[3] UNESP Univ Estadual Paulista, Inst Biociencias, Dept Bot, BR-13506900 Rio Claro - Brazil

^[4] J Craig Venter Inst, La Jolla, CA 92037 - USA

^[5] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agropecuaria, BR-14884900 Jaboticabal - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 1 JAN 1 2020.
• Citações Web of Science: 0

Resumo

Utricularia belongs to Lentibulariaceae, a widespread family of carnivorous plants that possess ultra-small and highly dynamic nuclear genomes. It has been shown that the Lentibulariaceae genomes have been shaped by transposable elements expansion and loss, and multiple rounds of whole-genome duplications (WGD), making the family a platform for evolutionary and comparative genomics studies. To explore the evolution of Utricularia, we estimated the chromosome number and genome size, as well as sequenced the terrestrial bladderwort Utricularia reniformis (2n = 40, 1C = 317.1-Mpb). Here, we report a high quality 304 Mb draft genome, with a scaffold NG50 of 466-Kb, a BUSCO completeness of 87.8%, and 42,582 predicted genes. Compared to the smaller and aquatic U. gibba genome (101 Mb) that has a 32% repetitive sequence, the U. reniformis genome is highly repetitive (56%). The structural differences between the two genomes are the result of distinct fractionation and rearrangements after WGD, and massive proliferation of LTR-retrotransposons. Moreover, GO enrichment analyses suggest an ongoing gene birth-death-innovation process occurring among the tandem duplicated genes, shaping the evolution of carnivory-associated functions. We also identified unique patterns of developmentally related genes that support the terrestrial life-form and body plan of U. reniformis. Collectively, our results provided additional insights into the evolution of the plastic and specialized Lentibulariaceae genomes. (AU)

• Processo FAPESP: 13/25164-6 - Genômica e transcriptoma de Utricularia reniformis (Lentibulariaceae): uma abordagem funcional e evolutiva
• Beneficiário: Alessandro de Mello Varani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/02285-6 - Genômica comparativa e evolução molecular de plantas carnívoras do gênero Utricularia (Lentibulariaceae)
• Beneficiário: Saura Rodrigues da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/05144-0 - Identificação e expressão de genes relacionados à pigmentação floral em Utricularia (Lentibulariaceae) com abordagem evolutiva
• Beneficiário: Vitor Fernandes Oliveira de Miranda
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/22215-3 - Respostas do genoma vegetal às variações ambientais: o que as Orchidaceae têm a contar?
• Beneficiário: Ana Paula de Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores