Role of GnRH Isoforms in Paracrine/Autocrine Control of Zebrafish (Danio rerio) Spermatogenesis

Autor(es)

Fallah, Hamideh P. ^[1] ; Rodrigues, Maira S. ^{[1, 2]} ; Corchuelo, Sheryll ^[2] ; Nobrega, Rafael H. ^[2] ; Habibi, Hamid R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Calgary, Dept Biol Sci, 2500 Univ Dr NW, Calgary, AB T2N 1N4 - Canada

^[2] Sao Paulo State Univ, Dept Morphol, Reprod & Mol Biol Grp, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Endocrinology; v. 161, n. 2 FEB 2020.
• Citações Web of Science: 0

Resumo

It is well established that hypothalamic GnRH (gonadotropin-releasing hormone) is one of the key peptides involved in the neuroendocrine control of testicular development and spermatogenesis. However, the role of GnRH as a paracrine regulator of testicular function has not been fully investigated. The present study demonstrates the presence of GnRH and its receptors in the zebrafish (Danio rerio) testis, and provides information on direct action of native GnRH isoforms (GnRH2 and GnRH3) on different stages of spermatogenesis in this model. Both GnRH2 and GnRH3 stimulated basal spermatogenesis by increasing numbers of type A(und) spermatogonia, spermatozoa, and testosterone release, and in this study GnRH2 exerted higher relative activity than GnRH3. Next, we evaluated the effects of GnRH isoforms on human chorionic gonadotropin (hCG)- and follicle-stimulating hormone (Fsh)-induced spermatogenesis. The 2 GnRH isoforms were found to have different effects on Fsh- and hCG-induced response depending on the stage of spermatogenesis and concentration of the peptides. The results provide strong support for the hypothesis that locally produced GnRH2 and GnRH3 are important components of the complex multifactorial system that regulates testicular germinal cell development and function in adult zebrafish. (AU)

• Processo FAPESP: 17/15793-7 - Efeitos do hipotireoidismo na função testicular de Danio rerio e relação dos hormônios tireoidianos com o hormônio folículo estimulante (Fsh)
• Beneficiário: Maira da Silva Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/15319-6 - Relação do hormônio inibidor de gonadotropina (GnIH) com os hormônios tireoidianos (HTs) no eixo hipotálamo-hipófise-periférico em zebrafish (Danio rerio)
• Beneficiário: Maira da Silva Rodrigues
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado