Scanning Electron Microscopy of Sand Flies of the Chagasi Series, Psychodopygus (Diptera: Psychodidae) Genus, Focusing on the Genitalia

Autor(es)

Godoy, Rodrigo Espindola ^{[1, 2]} ; Rangel, Elizabeth Ferreira ^[2] ; dos Santos Mallet, Jacenir Reis ^[2] ; dos Santos, Thiago Vasconcelos ^[3] ; Barata, Iorlando da Rocha ^[3] ; Costa Pessoa, Felipe Arley ^[4] ; Farias Lobato da Cunha, Alda Eunice ^[5] ; Vilela, Mauricio Luiz ^[2] ; Bianchi Galati, Eunice Aparecida ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01246904 Sao Paulo, SP - Brazil

^[2] Fiocruz MS, Inst Oswaldo Cruz, Lab Interdisciplinar Vigilancia Entomol Diptera &, BR-21040360 Rio De Janeiro - Brazil

^[3] Inst Evandro Chagas, Lab Leishmanioses, Rodovia BR-316, Km 7, BR-67030000 Ananindeua, PA - Brazil

^[4] Fundacao Oswaldo Cruz, Inst Leonidas & Maria Deane, Lab Ecol Doencas Transmissiveis Amazonia, Rua Teresina 476, BR-69057070 Manaus, Amazonas - Brazil

^[5] Secretaria Estadual Saude Rondonia, Lab Cent Saude Publ, R Anita Garibalde 4130, BR-76803620 Porto Velho, RO - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Entomology; v. 57, n. 1, p. 92-103, JAN 2020.
• Citações Web of Science: 0

Resumo

Males of cryptic or closely related species present great morphological variation in their genitalia, whereas females, such as those of the Chagasi Series of the Psychodopygus Mangabeira, 1941 genus, are more similar. Therefore, our aim was to study the fine structure of the male genitalia of five species of the Chagasi Series to better understand the variation in their morphology and its influence on the copulatory process. The sand fly species were captured in the following Brazilian states: Psychodopygus chagasi (Costa Lima, 1941) (Rondonia), Psychodopygus complexus (Mangabeira, 1941) (Tocantins), Psychodopygus squamiventris maripaensis (Floch \& Abonnenc, 1946) (Amapa), Psychodopygus squamiventris squamiventris (Lutz \& Neiva, 1912) (Amazonas), and Psychodopygus wellcomei Fraiha, Shaw \& Lainson, 1971 (Para and Ceara). Insects were stored in ethanol 70% (then dehydrated) and dry after they were sputtered with gold. The samples were observed under a scanning electron microscope. Microtrichiae, two types of trichoid sensilla, coeloconic and chaetic sensillae, were observed on the antenna of all species, with no difference between them. Only on the anepimeron of P. squamiventris squamiventris a modified `racket'-like scale was observed. As for the male genitalia, the setae and structures of each species were fully described, such as the small setae on the paramere apex of the P. squamiventris subspecies, and the grooves present in this region and on the paramere lobe of P. complexus and P. wellcomei, which are impossible to observe with optic microscopy. New information is thus provided on the male genitalia, which can contribute to future bionomic studies of these species. (AU)

• Processo FAPESP: 15/02282-9 - Estudo taxonômico de flebotomíneos do gênero Psychodopygus (Mangabeira, 1941) série chagasi (Barretto, 1962), através de análises morfométricas, morfológicas e barcoding
• Beneficiário: Rodrigo Espíndola Godoy
• Modalidade de apoio: Bolsas no Brasil - Doutorado