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Anticandidal activity of synthetic peptides: Mechanism of action revealed by scanning electron and fluorescence microscopies and synergism effect with nystatin

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Autor(es):
Lima, Patricia G. [1] ; Souza, Pedro F. N. [1] ; Freitas, Cleverson D. T. [1] ; Oliveira, Jose T. A. [1] ; Dias, Lucas P. [1] ; Neto, Joao X. S. [1] ; Vasconcelos, Ilka M. [1] ; Lopes, Jose L. S. [2] ; Sousa, Daniele O. B. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Fed Ceara, Dept Biochem & Mol Biol, Fortaleza, Ceara - Brazil
[2] Univ Sao Paulo, Dept Appl Phys, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PEPTIDE SCIENCE; v. 26, n. 6 MAR 2020.
Citações Web of Science: 0
Resumo

Candida albicans has emerged as a major public health problem in recent decades. The most important contributing factor is the rapid increase in resistance to conventional drugs worldwide. Synthetic antimicrobial peptides (SAMPs) have attracted substantial attention as alternatives and/or adjuvants in therapeutic treatments due to their strong activity at low concentrations without apparent toxicity. Here, two SAMPs, named Mo-CBP3-PepI (CPAIQRCC) and Mo-CBP3-PepII (NIQPPCRCC), are described, bioinspired by Mo-CBP3, which is an antifungal chitin-binding protein from Moringa oleifera seeds. Furthermore, the mechanism of anticandidal activity was evaluated as well as their synergistic effects with nystatin. Both peptides induced the production of reactive oxygen species (ROS), cell wall degradation, and large pores in the C. albicans cell membrane. In addition, the peptides exhibited high potential as adjuvants because of their synergistic effects, by increasing almost 50-fold the anticandidal activity of the conventional antifungal drug nystatin. These peptides have excellent potential as new drugs and/or adjuvants to conventional drugs for treatment of clinical infections caused by C. albicans. (AU)

Processo FAPESP: 18/19546-7 - Mecanismos moleculares da ligação, inserção e orientação de peptídeos antimicrobianos em modelos de membrana
Beneficiário:Jose Luiz de Souza Lopes
Modalidade de apoio: Auxílio à Pesquisa - Regular