A Nanomechanical Genosensor Using Functionalized Cantilevers to Detect the Cancer Biomarkers miRNA-203 and miRNA-205

Autor(es)

Andrade, Mariana A. ^[1] ; Rodrigues, Livia F. ^[1] ; Lerich, Jessica C. M. ^[1] ; Melendez, Matias E. ^[2] ; Carvalho, Andre L. ^[2] ; de Carvalho, Ana C. ^[2] ; Steffens, Clarice ^[3] ; Abdalla, Fabio C. ^[4] ; Oliveira, Jr., Osvaldo N. ^[5] ; Leite, Fabio L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Phys Chem & Math, Nanoneurobiophys Res Grp, BR-18052780 Sorocaba - Brazil

^[2] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos - Brazil

^[3] Reg Integrated Univ High Uruguay & Missions, Dept Food Engn, BR-99700000 Erechim - Brazil

^[4] Univ Fed Sao Carlos, Dept Biol, Lab Struct & Funct Biol, BR-18052780 Sorocaba - Brazil

^[5] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: IEEE SENSORS JOURNAL; v. 20, n. 6, p. 2860-2867, MAR 15 2020.
• Citações Web of Science: 0

Resumo

We report a nanomechanical genosensor based on an atomic force microscope (AFM) cantilever functionalized with complementary strands of miRNA-203 and miRNA-205, which are potential biomarkers of metastasis in head and neck squamous cell carcinomas (HNSCC), especially in cervical lymph nodes. Hybridization with the corresponding complementary strand led to cantilever deflections that increased almost linearly with the concentration of either miRNA-203 or miRNA-205 in the fluid cell where AFM experiments were made. The detection limit was ca. 95 nM and 97 nM for miRNA-203 or miRNA-205, respectively, with higher deflections observed for miRNA-205 due to the larger number of interaction groups during hybridization. Selectivity was proven by measuring negligible cantilever deflections for non-specific miRNA sequences. The cantilever functionalization process during genosensor design was confirmed by fluorescence microscopy and AFM imaging. The use of a single genosensor platform for detecting different miRNA molecules is advantageous for diagnosis and prognosis of cases where multiple biomarkers may be relevant, as in the case of HNSCC metastasis. (AU)

• Processo FAPESP: 14/50869-6 - INCT 2014: em Eletrônica Orgânica INEO
• Beneficiário: Roberto Mendonça Faria
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/12082-4 - Modelagem molecular das interações do complexo antígeno-anticorpo na investigação de doenças desmielinizantes autoimunes
• Beneficiário: Jéssica Cristiane Magalhães Ierich
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/25549-0 - Aplicação de sensores nanomecânicos de cantilever para detecção de metástases de carcinoma epidermóide de cabeça e pescoço (CECP)
• Beneficiário: Mariana Arnaud de Andrade Bortolini
• Modalidade de apoio: Bolsas no Brasil - Mestrado