Butanol production by Saccharomyces cerevisiae: perspectives, strategies and challenges

Autor(es)

Azambuja, Suellen P. H. ^[1] ; Goldbeck, Rosana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Engn, Lab Bioproc & Metab Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 36, n. 3 MAR 9 2020.
• Citações Web of Science: 1

Resumo

The search for gasoline substitutes has grown in recent decades, leading to the increased production of ethanol as viable alternative. However, research in recent years has shown that butanol exhibits various advantages over ethanol as a biofuel. Furthermore, butanol can also be used as a chemical platform, serving as an intermediate product and as a solvent in industrial reactions. This alcohol is naturally produced by some Clostridium species; however, Clostridial fermentation processes still have inherent problems, which focuses the interest on Saccharomyces cerevisiae for butanol production, as an alternative organism for the production of this alcohol. S. cerevisiae exhibits great adaptability to industrial conditions and can be modified with a wide range of genetic tools. Although S. cerevisiae is known to naturally produce isobutanol, the n-butanol synthesis pathway has not been well established in wild S. cerevisiae strains. Two strategies are most commonly used for of S. cerevisiae butanol production: the heterologous expression of the Clostridium pathway or the amino acid uptake pathways. However, butanol yields produced from S. cerevisiae are lower than ethanol yield. Thus, there are still many challenges needed to be overcome, which can be minimized through genetic and evolutive engineering, for butanol production by yeast to become a reality. (AU)

• Processo FAPESP: 19/08542-3 - Biotecnologia enzimática para produção de xilo-oligossacarídeos a partir de resíduos agroindustriais lignocelulósicos visando a produção de simbióticos
• Beneficiário: Rosana Goldbeck Coelho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
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• Modalidade de apoio: Auxílio à Pesquisa - Temático