Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)

Autor(es)

Godoi, Filipe G. A. ^[1] ; Munoz-Penuela, Marcela ^[1] ; Olio Gomes, Aline D. ^[1] ; Tolussi, Carlos E. ^[2] ; Brambila-Souza, Gabriela ^[3] ; Branco, Giovana S. ^[3] ; Lo Nostro, Fabiana L. ^{[4, 5]} ; Moreira, Renata G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Rua Matao, Trav 14, 321, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Anhembi Morumbi, Campus Mooca, Sao Paulo, SP - Brazil

^[3] Univ Estadual Paulista, Ctr Aquicultura CAUNESP, UNESP, Jaboticabal, SP - Brazil

^[4] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, Lab Ecotoxicol Acuat, Ciudad Univ C1428EHA, Buenos Aires, DF - Argentina

^[5] CONICET UBA, IBBEA, Ciudad Univ C1428EHA, Buenos Aires, DF - Argentina

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 231, MAY 2020.
• Citações Web of Science: 0

Resumo

Diclofenac (DCF) and caffeine (CAF) are persistent pharmaceuticals that occur in mixtures in the aquatic ecosystems causing effects in the reproductive physiology of aquatic organisms. This study evaluated the physiological reproductive responses of Astyanax altiparanae males exposed to nominal concentrations of DCF (3.08 mg L-1) and CAF (9.59 mg L-1) separately and combined, for 96 h. The steroids profile, estrogenic biomarker vitellogenin (vtgA), testes and liver morphology, and also mortality of males were assessed. DCF and CAF degradation was 5% of the initial concentration for 24 h. The LC50 of the DCF and CAF were 30.8 mg L-1 and 95.9 mg L-1 , respectively. Males exposed to DCF and CAF exhibited a reduction of 175-Estradiol (E-2) concentration compared to control (CTL). Similarly, testosterone (T) was also reduced in the DCF treatment, but this response was not observed in 11-Ketotestosterone (11-KT). Males exposed to DCF + CAF combined did not exhibit differences in T, E-2 and 11-KT steroids. The vtgA gene expression and the sperm concentration did not change among the treatments. Moreover, acute exposure revealed a hypertrophy of hepatocytes cells in the DCF and DCF + CAF treatments. In conclusion, DCF and CAF, isolated, exhibit an endocrine disruptive activity in A. altiparanae male, an opposite response observed with the mixture of both compounds that abolishes the endocrine disruptive effects. DCF seems to be more toxic for this species, altering also hepatocytes morphology. (AU)

• Processo FAPESP: 17/07139-5 - Ajustes na fisiologia reprodutiva de machos Astyanax altiparanae (Teleostei: Characidae) expostos a contaminantes emergentes
• Beneficiário: Filipe Guilherme Andrade de Godoi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/16320-7 - Impactos das mudanças climáticas e ambientais sobre a fauna: uma abordagem integrativa
• Beneficiário: Carlos Arturo Navas Iannini
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático
• Processo FAPESP: 17/11530-1 - Ajustes fisiológicos de teleósteos tropicais em reservatórios impactados
• Beneficiário: Renata Guimarães Moreira Whitton
• Modalidade de apoio: Auxílio à Pesquisa - Regular