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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae)

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Author(s):
Godoi, Filipe G. A. [1] ; Munoz-Penuela, Marcela [1] ; Olio Gomes, Aline D. [1] ; Tolussi, Carlos E. [2] ; Brambila-Souza, Gabriela [3] ; Branco, Giovana S. [3] ; Lo Nostro, Fabiana L. [4, 5] ; Moreira, Renata G. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Rua Matao, Trav 14, 321, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ Anhembi Morumbi, Campus Mooca, Sao Paulo, SP - Brazil
[3] Univ Estadual Paulista, Ctr Aquicultura CAUNESP, UNESP, Jaboticabal, SP - Brazil
[4] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, Lab Ecotoxicol Acuat, Ciudad Univ C1428EHA, Buenos Aires, DF - Argentina
[5] CONICET UBA, IBBEA, Ciudad Univ C1428EHA, Buenos Aires, DF - Argentina
Total Affiliations: 5
Document type: Journal article
Source: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 231, MAY 2020.
Web of Science Citations: 0
Abstract

Diclofenac (DCF) and caffeine (CAF) are persistent pharmaceuticals that occur in mixtures in the aquatic ecosystems causing effects in the reproductive physiology of aquatic organisms. This study evaluated the physiological reproductive responses of Astyanax altiparanae males exposed to nominal concentrations of DCF (3.08 mg L-1) and CAF (9.59 mg L-1) separately and combined, for 96 h. The steroids profile, estrogenic biomarker vitellogenin (vtgA), testes and liver morphology, and also mortality of males were assessed. DCF and CAF degradation was 5% of the initial concentration for 24 h. The LC50 of the DCF and CAF were 30.8 mg L-1 and 95.9 mg L-1 , respectively. Males exposed to DCF and CAF exhibited a reduction of 175-Estradiol (E-2) concentration compared to control (CTL). Similarly, testosterone (T) was also reduced in the DCF treatment, but this response was not observed in 11-Ketotestosterone (11-KT). Males exposed to DCF + CAF combined did not exhibit differences in T, E-2 and 11-KT steroids. The vtgA gene expression and the sperm concentration did not change among the treatments. Moreover, acute exposure revealed a hypertrophy of hepatocytes cells in the DCF and DCF + CAF treatments. In conclusion, DCF and CAF, isolated, exhibit an endocrine disruptive activity in A. altiparanae male, an opposite response observed with the mixture of both compounds that abolishes the endocrine disruptive effects. DCF seems to be more toxic for this species, altering also hepatocytes morphology. (AU)

FAPESP's process: 17/07139-5 - Adjustments on males reproductive physiology of Astyanax altiparanae (Teleostei:Characidae) exposed to emerging compounds
Grantee:Filipe Guilherme Andrade de Godoi
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach
Grantee:Carlos Arturo Navas Iannini
Support Opportunities: Research Program on Global Climate Change - Thematic Grants
FAPESP's process: 17/11530-1 - Physiological adjustments of tropical teleosts in anthropogenic reservoirs
Grantee:Renata Guimarães Moreira Whitton
Support Opportunities: Regular Research Grants