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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Anticancer properties of the fatty acid synthase inhibitor TVB-3166 on oral squamous cell carcinoma cell lines

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Autor(es):
de Aquino, Iara Goncalves [1] ; Bastos, Debora Campanella [1, 2] ; Maria Cuadra-Zelaya, Florence Juana [1] ; Teixeira, Isadora Ferrari [1] ; Salo, Tuula [3, 4, 5, 6] ; Della Coletta, Ricardo [1] ; Graner, Edgard [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] State Univ Campinas UNICAMP, Sch Dent Piracicaba, Dept Oral Diag, Av Limeira 901, BR-13414018 Piracicaba, SP - Brazil
[2] State Univ Campinas UNICAMP, Sch Dent Piracicaba, Dept Morphol, Piracicaba, SP - Brazil
[3] Univ Helsinki, Inst Oral & Maxillofacial Dis, Helsinki - Finland
[4] Helsinki Univ Hosp, HUSLAB, Dept Pathol, Helsinki - Finland
[5] Univ Oulu, Oulu Univ Hosp, Fac Med, Canc & Translat Med Res Unit, Oulu - Finland
[6] Univ Oulu, Med Res Ctr Oulu, Oulu Univ Hosp, Oulu - Finland
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: ARCHIVES OF ORAL BIOLOGY; v. 113, MAY 2020.
Citações Web of Science: 0
Resumo

Objective: Fatty acid synthase (FASN) is overexpressed in several human cancers, including oral squamous cell carcinoma (OSCC). TVB-3166 is a recently described FASN inhibitor with antitumor effects and potential clinical relevance. The objective of this study was to evaluate the effects of TVB-3166 on OSCC cell lines. Materials and methods: The OSCC cell line SCC-9 modified to express ZsGreen (ZsG) (SCC-9 ZsG) and its in vivo selected metastatic derivative LN-1A were used to evaluate anticancer properties of TVB-3166. Cell viability was determined using MTT assays and proliferation determined by cell counting in a Neubauer chamber. Cell death and cell cycle progression were analyzed by Annexin V-PE/7-ADD-PerCP labeling and PI staining, respectively. Cell migration was assayed by scratch assays and cell adhesion using myogel. Production of FASN, p-AKT, CPT1-alpha, and epithelial-mesenchymal transition (EMT) markers were examined by Western blotting. Results: TVB-3166 significantly reduced cell viability and proliferation, promoted cell cycle arrest and apoptosis, and increased adhesion to myogel in both OSCC cell lines. Finally, the drug reduced SCC-9 ZsG migration. Conclusion: Our results demonstrated that TVB-3166 has anticancer effects on both SCC-9 ZsG and its metastatic version LN-1A, which are worthy of investigation in preclinical models for OSCC. (AU)

Processo FAPESP: 14/20832-3 - Efeitos da associação de Orlistat com agentes quimioterápicos em carcinoma espinocelular de língua: estudo in vitro e em modelos ortotópicos
Beneficiário:Edgard Graner
Modalidade de apoio: Auxílio à Pesquisa - Regular