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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Anticancer properties of the fatty acid synthase inhibitor TVB-3166 on oral squamous cell carcinoma cell lines

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de Aquino, Iara Goncalves [1] ; Bastos, Debora Campanella [1, 2] ; Maria Cuadra-Zelaya, Florence Juana [1] ; Teixeira, Isadora Ferrari [1] ; Salo, Tuula [3, 4, 5, 6] ; Della Coletta, Ricardo [1] ; Graner, Edgard [1]
Total Authors: 7
[1] State Univ Campinas UNICAMP, Sch Dent Piracicaba, Dept Oral Diag, Av Limeira 901, BR-13414018 Piracicaba, SP - Brazil
[2] State Univ Campinas UNICAMP, Sch Dent Piracicaba, Dept Morphol, Piracicaba, SP - Brazil
[3] Univ Helsinki, Inst Oral & Maxillofacial Dis, Helsinki - Finland
[4] Helsinki Univ Hosp, HUSLAB, Dept Pathol, Helsinki - Finland
[5] Univ Oulu, Oulu Univ Hosp, Fac Med, Canc & Translat Med Res Unit, Oulu - Finland
[6] Univ Oulu, Med Res Ctr Oulu, Oulu Univ Hosp, Oulu - Finland
Total Affiliations: 6
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 113, MAY 2020.
Web of Science Citations: 0

Objective: Fatty acid synthase (FASN) is overexpressed in several human cancers, including oral squamous cell carcinoma (OSCC). TVB-3166 is a recently described FASN inhibitor with antitumor effects and potential clinical relevance. The objective of this study was to evaluate the effects of TVB-3166 on OSCC cell lines. Materials and methods: The OSCC cell line SCC-9 modified to express ZsGreen (ZsG) (SCC-9 ZsG) and its in vivo selected metastatic derivative LN-1A were used to evaluate anticancer properties of TVB-3166. Cell viability was determined using MTT assays and proliferation determined by cell counting in a Neubauer chamber. Cell death and cell cycle progression were analyzed by Annexin V-PE/7-ADD-PerCP labeling and PI staining, respectively. Cell migration was assayed by scratch assays and cell adhesion using myogel. Production of FASN, p-AKT, CPT1-alpha, and epithelial-mesenchymal transition (EMT) markers were examined by Western blotting. Results: TVB-3166 significantly reduced cell viability and proliferation, promoted cell cycle arrest and apoptosis, and increased adhesion to myogel in both OSCC cell lines. Finally, the drug reduced SCC-9 ZsG migration. Conclusion: Our results demonstrated that TVB-3166 has anticancer effects on both SCC-9 ZsG and its metastatic version LN-1A, which are worthy of investigation in preclinical models for OSCC. (AU)

FAPESP's process: 14/20832-3 - The association of Orlistat with chemotherapic agents for the treatment of oral squamous cell carcinoma: an in vitro and in vivo study in orthotopic models
Grantee:Edgard Graner
Support Opportunities: Regular Research Grants