| Texto completo | |
| Autor(es): |
dos Santos, Taina Caroline
[1]
;
Sousa, Kaline de Brito
[1]
;
Andreo, Lucas
[1]
;
Martinelli, Andreia
[2]
;
Setubal Destro Rodrigues, Maria Fernanda
[1]
;
Bussadori, Sandra Kalil
[1, 2]
;
Santos Fernandes, Kristianne Porta
[1]
;
Mesquita-Ferrari, Raquel Agnelli
[1, 2]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil
[2] UNINOVE, Postgrad Program Rehabil Sci, Sao Paulo, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Photochemistry and Photobiology; v. 96, n. 4 APR 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Moderate levels of a proinflammatory macrophages phenotype are indispensable and play an important role in the skeletal muscle repair process since this response depends on their secreted products concentration to influence and modulate muscle inflammation as well as the differentiation of myoblasts. This study investigated the effects of photobiomodulation (PBM) on undifferentiated and differentiation-induced C2C12 myoblasts cultivated in different concentrations of M1 phenotype macrophage-conditioned media of J774 cells (MCM1) also submitted to PBM using the same irradiation parameters. Irradiation was performed once with low-level laser (780 nm, 70 mW, 1 J) and was evaluated cell viability, proliferation and differentiation, nitric oxide (NO) synthesis and IL-6 and TNF-alpha protein levels 24 and 48 h after C2C12 irradiation. PBM treatment in undifferentiated myoblasts exhibited lower IL-6 levels in the presence of nonirradiated MCM1 at both concentrations. Myoblasts in proliferation condition cultivated with irradiated MCM1 showed lower IL-6 and TNF-alpha levels after 48 h in the presence of both concentrations evaluated. PBM induced a decrease in the synthesis of NO on undifferentiated and differentiation-induced myoblasts. PBM was able to reduce the level of proinflammatory protein and markers, which are important to allow the differentiation of myoblasts during the muscle repair process. (AU) | |
| Processo FAPESP: | 14/12381-1 - Análise comparativa do efeito da laserterapia prévia associada ou não ao tratamento pós sobre a modulação de citocinas inflamatórias, do estresse oxidativo e a determinação do tipo de fibra muscular após lesão aguda |
| Beneficiário: | Raquel Agnelli Mesquita Ferrari |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |