Telomere Length and Telomerase Activity in Immature Oocytes and Cumulus Cells of Women with Polycystic Ovary Syndrome

Autor(es):Mostrar menos -

Pedroso, Daiana C. C. ^[1] ; Santana, Viviane P. ^[1] ; Donaires, Flavia S. ^[2] ; Picinato, Maria C. ^[1] ; Giorgenon, Roberta C. ^[1] ; Santana, Barbara A. ^[2] ; Pimentel, Ricardo N. ^[1] ; Keefe, David L. ^[3] ; Calado, Rodrigo T. ^[2] ; Ferriani, Rui A. ^[1] ; Furtado, Cristiana L. M. ^{[1, 4]} ; Reis, Rosana M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Med Imaging Hematol & Clin Oncol, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] NYU, Sch Med, Dept Obstet & Gynecol, New York, NY - USA

^[4] Univ Fed Ceara, Drug Res & Dev Ctr, Postgrad Program Med & Surg Sci, BR-60430275 Fortaleza, Ceara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTIVE SCIENCES; v. 27, n. 6 JAN 2020.
• Citações Web of Science: 0

Resumo

Metaphase II oocytes (MII) from polycystic ovary syndrome (PCOS) frequently have impaired oocyte competence. Since telomere maintenance is important for folliculogenesis, oocyte maturation, and early embryonic development, we sought to verify the implications of PCOS on telomere length and telomerase activity in immature oocytes and cumulus cells. 43 PCOS and 67 control women were included, and anthropometric, biochemical, and hormonal characteristics were evaluated. The telomere length in germinal vesicle stage (GV) and in metaphase I (MI) oocytes, as well as in the cumulus cells of immature (CCI) and mature oocytes (CCM), and in leukocytes was measured by qPCR. The telomerase activity in reproductive cells was evaluated by the TRAPeze (R) XL Kit. The body mass index (p = 0.001), LH (p = 0.015), estradiol (p = 0.004), insulin (p = 0.002), testosterone (p < 0.0001), androstenedione (p = 0.001), free androgen index (p < 0.0001), and c-reactive protein (p = 0.003) were greater, while the FSH (p = 0.0002) was lower in the PCOS group. The telomere length in the CCI (p = 0.649) and CCM (p = 0.378) did not differ between the PCOS and the control groups. On the other hand, telomerase activity in the CCI (p = 0.003) and CCM (p = 0.022) was higher in the PCOS group. In the leukocyte's cells, the telomere length was reduced in the PCOS group (p = 0.025). In the GV and MI oocytes, no differences were observed in telomere length and telomerase activity between the groups. We showed that telomere length is not altered in reproductive cells from PCOS. However, higher telomerase activity in the CCI and CCM may be required for telomere length maintenance. (AU)

• Processo FAPESP: 12/11566-2 - Polimorfismo cag do receptor de andrógenos e comprimento do telômero em pacientes com falência ovariana prematura
• Beneficiário: Rosana Maria dos Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/11069-9 - Polimorfismo cag do receptor de andrógenos e comprimento do telômero em pacientes com falência ovariana prematura
• Beneficiário: Cristiana Libardi Miranda Furtado
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado