Dietary methionine level alters growth, digestibility, and gene expression of amino acid transporters in meat-type chickens

Autor(es)

Fagundes, Naiara S. ^{[1, 2]} ; Milfort, Marie C. ^[2] ; Williams, Susan M. ^[3] ; Da Costa, Manuel J. ^[2] ; Fuller, Alberta L. ^[2] ; Menten, Jose F. ^[1] ; Rekaya, Romdhane ^[4] ; Aggrey, Samuel E. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anim Sci, Piracicaba, SP - Brazil

^[2] Univ Georgia, Dept Poultry Sci, NutriGen Lab, Athens, GA 30602 - USA

^[3] Univ Georgia, Dept Populat Hlth, Athens, GA 30602 - USA

^[4] Univ Georgia, Dept Anim & Dairy Sci, Athens, GA 30602 - USA

• Tipo de documento: Artigo Científico
• Fonte: POULTRY SCIENCE; v. 99, n. 1, p. 67-75, JAN 2020.
• Citações Web of Science: 0

Resumo

Imbalance in nutrients can affect digestibility of amino acids by altering gene expression of amino acid transporters. We investigated digestibility and molecular transporters of essential amino acids in chickens fed a methionine-deficient diet. A total of 40 chicks (23 D old) were randomly assigned to either a control (0.49% methionine) or a deficient (0.28%) diet until 41 D when they were sampled for Pectoralis (P.) major, kidney, ileum, and hypothalamus for mRNA expression analysis. The ileal content was collected for apparent ileal digestibility (AID) analysis. Birds fed the deficient diet had reduced growth and worse feed efficiency compared to control. The AID of methionine was similar between both groups. The AID of other essential amino acids was higher in the deficient group than control. mRNA expression of b(0)+AT and LAT4 were upregulated in the ileum and kidney but LAT1 was downregulated only in kidney of the deficient group compared to control. In the P. major, SNAT1, SNAT2, and CAT1 were upregulated in the deficient group compared to control. A diet deficiency in methionine affects digestibility of essential amino acids and cysteine, but not the digestibility of methionine. The change in digestibility is reflected in the mRNA expression of amino acid transporters across different tissues. (AU)

• Processo FAPESP: 15/00656-9 - Efeito da metionina dietética na partição de nutrientes no íleo e transportadores de aminoácidos
• Beneficiário: Naiara Simarro Fagundes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado