Arachidonic acid effects on the overall performance, fatty acid profile, hepatopancreas morphology and lipid-relevant genes in Litopenaeus vannamei juveniles

Autor(es)

Araujo, Bruno Cavalheiro ^{[1, 2]} ; Flores-Galvez, Krishna ^[3] ; Honji, Renato Massaaki ^[2] ; Magalhaes Barbosa, Vitalina ^[3] ; Teresa Viana, Maria ^[3] ; Tinajero, Aurora ^[3] ; Antonio Mata-Sotres, Jose ^[4]

Afiliação do(s) autor(es)

^[1] Univ Mogi das Cruzes, Nucleo Integrad Biotecnol, Av Dr Candido Xavier de Almeida Souza 200, BR-08780911 Mogi Das Cruzes, SP - Brazil

^[2] Univ Sao Paulo, Ctr Biol Marinha, CEBIMar, Rodovia Manoel Hipolito do Rego, Km 131-5, BR-11612109 Sao Sebastiao, SP - Brazil

^[3] Univ Autonoma Baja California, Inst Invest Oceanol, Km 107 Carretera Tij Eda, Ensenada 22860, Baja California - Mexico

^[4] CONACYT, UABC, Inst Invest Oceanol, Ensenada, Baja California - Mexico

• Tipo de documento: Artigo Científico
• Fonte: Aquaculture; v. 523, JUN 30 2020.
• Citações Web of Science: 0

Resumo

The effect of arachidonic acid (ARA) was evaluated on Litopenaeus vannamei juveniles. Growth performance; hepatopancreas and muscle fatty acid compositions; hepatopancreatic cells morphology; and expression of lipid-relevant genes were estimated after six-week experiment. A basal fat-reduced diet was manufactured and subsequently coated with different fatty acids sources, distinct levels of ARA (0, 0.3, and 0.6%), fish and soybean oil (50:50 Control diet). L. vannamei juveniles (1.10 +/- 0.22 g) were randomly divided into twelve tanks with thirty animals each. The animals were fed three times per day, with 8% of the total biomass. The results showed that animals fed with diet containing 0.3% of ARA presented a lower final weight compared to the Control. Different levels of ARA inclusion in the experimental diets directly influenced in the tissues fatty acid profile with lower ARA levels in Control and 0% followed by 0.3% and 0.6%. In muscle and hepatopancreas tissues, a higher accumulation of docosahexaenoic acid (DHA) was revealed in the Control compared to the rest of the experimental treatments. The expression pattern of lipoxygenase (alox5), prostaglandin E synthase (pges2), sterol regulatory element-binding protein-1 (srebp1) and cycloxygenase (cox2) were significantly affected by the ARA inclusion, showing the highest expression in the Control treatment with a decrease according to the increasing levels of dietary ARA. It is concluded that ARA inclusion in shrimp diets result in a negative impact on the overall performance, probably modulated by a differential gene expression related to the eicosanoids synthesis, lipolytic and lipogenic pathways. Besides, the lipid deposition on the hepatopancreatic cells was reduced. (AU)

• Processo FAPESP: 17/06765-0 - Fisiologia reprodutiva da Garoupa-Verdadeira Epinephelus marginatus (Perciformes: Serranidae) em cativeiro: terapias hormonais aplicadas à indução da maturação sexual na fase pré-desova
• Beneficiário: Renato Massaaki Honji
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/13000-2 - Substituição de subprodutos de peixe por fontes lipídicas alternativas suplementadas com LC-PUFA n3 em dietas específicas para peixes marinhos
• Beneficiário: Bruno Cavalheiro Araújo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado