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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Arachidonic acid effects on the overall performance, fatty acid profile, hepatopancreas morphology and lipid-relevant genes in Litopenaeus vannamei juveniles

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Author(s):
Araujo, Bruno Cavalheiro [1, 2] ; Flores-Galvez, Krishna [3] ; Honji, Renato Massaaki [2] ; Magalhaes Barbosa, Vitalina [3] ; Teresa Viana, Maria [3] ; Tinajero, Aurora [3] ; Antonio Mata-Sotres, Jose [4]
Total Authors: 7
Affiliation:
[1] Univ Mogi das Cruzes, Nucleo Integrad Biotecnol, Av Dr Candido Xavier de Almeida Souza 200, BR-08780911 Mogi Das Cruzes, SP - Brazil
[2] Univ Sao Paulo, Ctr Biol Marinha, CEBIMar, Rodovia Manoel Hipolito do Rego, Km 131-5, BR-11612109 Sao Sebastiao, SP - Brazil
[3] Univ Autonoma Baja California, Inst Invest Oceanol, Km 107 Carretera Tij Eda, Ensenada 22860, Baja California - Mexico
[4] CONACYT, UABC, Inst Invest Oceanol, Ensenada, Baja California - Mexico
Total Affiliations: 4
Document type: Journal article
Source: Aquaculture; v. 523, JUN 30 2020.
Web of Science Citations: 0
Abstract

The effect of arachidonic acid (ARA) was evaluated on Litopenaeus vannamei juveniles. Growth performance; hepatopancreas and muscle fatty acid compositions; hepatopancreatic cells morphology; and expression of lipid-relevant genes were estimated after six-week experiment. A basal fat-reduced diet was manufactured and subsequently coated with different fatty acids sources, distinct levels of ARA (0, 0.3, and 0.6%), fish and soybean oil (50:50 Control diet). L. vannamei juveniles (1.10 +/- 0.22 g) were randomly divided into twelve tanks with thirty animals each. The animals were fed three times per day, with 8% of the total biomass. The results showed that animals fed with diet containing 0.3% of ARA presented a lower final weight compared to the Control. Different levels of ARA inclusion in the experimental diets directly influenced in the tissues fatty acid profile with lower ARA levels in Control and 0% followed by 0.3% and 0.6%. In muscle and hepatopancreas tissues, a higher accumulation of docosahexaenoic acid (DHA) was revealed in the Control compared to the rest of the experimental treatments. The expression pattern of lipoxygenase (alox5), prostaglandin E synthase (pges2), sterol regulatory element-binding protein-1 (srebp1) and cycloxygenase (cox2) were significantly affected by the ARA inclusion, showing the highest expression in the Control treatment with a decrease according to the increasing levels of dietary ARA. It is concluded that ARA inclusion in shrimp diets result in a negative impact on the overall performance, probably modulated by a differential gene expression related to the eicosanoids synthesis, lipolytic and lipogenic pathways. Besides, the lipid deposition on the hepatopancreatic cells was reduced. (AU)

FAPESP's process: 17/06765-0 - Reproductive physiology of the Dusky Grouper Epinephelus marginatus (Perciformes: Serranidae) in captivity: hormonal therapies applied to the induction of sexual maturation in pre-spawning phase
Grantee:Renato Massaaki Honji
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 18/13000-2 - Replacement of fish by-products by alternative lipid sources supplemented with n3 LC-PUFAs in specific diets for marine fish species
Grantee:Bruno Cavalheiro Araújo
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor