Sparger design as key parameter to define shear conditions in pneumatic bioreactors

Autor(es)

Esperanca, Mateus N. ^[1] ; Mendes, Caroline E. ^[2] ; Rodriguez, Guilherme Y. ^[3] ; Cerri, Marcel O. ^[4] ; Bettega, Rodrigo ^[5] ; Badino, Alberto C. ^[5]

Afiliação do(s) autor(es)

^[1] Fed Inst Educ Sci & Technol Sao Paulo, Campus Capivari, BR-13360000 Capivari, SP - Brazil

^[2] Fed Univ Rio Grande, Chem & Food Sch, Carreiros, RS - Brazil

^[3] Univ Fed Itajuba, Nat Resources Inst, Bioproc Engn Course, Itajuba, MG - Brazil

^[4] Sao Paulo State Univ, Fac Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara, SP - Brazil

^[5] Univ Fed Sao Carlos, Grad Program Chem Engn, CP 676, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biochemical Engineering Journal; v. 157, APR 15 2020.
• Citações Web of Science: 0

Resumo

The average shear rate (<(gamma)over dot>(av)) is a parameter used to characterize the shear environment in bioreactors, enabling comparison of the performances of different bioreactor models in terms of microorganism morphology and viability, and consequently bioproduct formation. Based on this approach, pneumatic bioreactors have been classified as low shear devices. However, the shear behavior cannot be generalized over a wide range of operating conditions, suggesting that the maximum shear rate (<(gamma)over dot>(max)) may be more suitable for the purpose of bioreactor performance comparison. Therefore, the aim of this work was to evaluate average and maximum shear rates in pneumatic bioreactors (bubble column and airlift), based on computational fluid dynamics (CFD) simulations. Concentric-duct and split airlift bioreactors exhibited higher <(gamma)over dot>(av) values, compared to the bubble column design, due to the nature of the liquid circulation patterns. The pneumatic bioreactors exhibited a significant (order of magnitude) difference between <(gamma)over dot>(av) (11.0-27.3 s(-1)) and <(gamma)over dot>(max) 4555 to 25,040 s(-1)), reflecting a non-uniform spatial distribution. The <(gamma)over dot>(max) values occurred close to the sparger holes and presented a linear relationship with gas injection velocity, which is dependent on the sparger geometry. In this way, sparger characteristics (number and diameter of sparger holes) defined <(gamma)over dot>(max) values in pneumatic bioreactors, showing that sparger should be properly designed in order to avoid excessive local shear rates. (AU)

• Processo FAPESP: 11/23807-1 - Desenvolvimento e avaliação das condições de operação de biorreatores visando a produção de produtos biotecnológicos
• Beneficiário: Alberto Colli Badino Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17756-8 - Avaliação das condições hidrodinâmicas, de cisalhamento e de transferência de oxigênio de diferentes modelos de biorreatores pneumáticos
• Beneficiário: Caroline Eliza Mendes
• Modalidade de apoio: Bolsas no Brasil - Doutorado