Correlation of plasma butyrylcholinesterase concentration with Acethylcholinesterase H353N polymorphism in the inflammatory response of Chagas disease patients

Autor(es)

Campello, A. C. ^[1] ; Zanella, L. G. F. A. B. D. E. ^{[2, 3]} ; Suzuki, R. B. ^{[1, 4]} ; Tokumo, M. O. ^[5] ; Chagas, E. F. B. ^[6] ; Baleotti, W. ^[7] ; Speranca, M. A. ^[8] ; Martins, L. P. A. ^[1]

Afiliação do(s) autor(es)

^[1] Marilia Med Sch, Dept Parasitol, Monte Carmelo Ave 800, BR-17519030 Marilia, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, BR-01246903 Sao Paulo, SP - Brazil

^[3] Servidor Publ Hosp, BR-04038034 Sao Paulo, SP - Brazil

^[4] Marilia Univ, Sch Med, BR-17525902 Marilia, SP - Brazil

^[5] Marilia Med Sch, Dept Cardiol, BR-17519030 Marilia, SP - Brazil

^[6] Marilia Univ, Study Grp Aging & Obes GEEO, BR-17525902 Marilia, SP - Brazil

^[7] Marilia Med Sch, Dept Clin Pathol, BR-17519030 Marilia, SP - Brazil

^[8] Fed Univ ABC, Ctr Nat & Human Sci, BR-09606070 Sao Bernardo Do Campo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology International; v. 76, JUN 2020.
• Citações Web of Science: 0

Resumo

Trypanosoma cruzi infection triggers an intense production of pro-inflammatory cytokines mediated by T helper 1 lymphocytes, inducing the anti-inflammatory reflex of acetylcholine (ACh). The ACh concentration modulation is associated to the two major esterases, the acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). AChE H353N protein polymorphism is related to low Chagas chronic disease prognostic. In order to evaluate the correlation of plasmatic BuChE concentration and the presence of AChE H353N polymorphism in Chagas disease patients and healthy individuals, we studied two groups of individuals, one of 61 Chagas disease patients and another of 74 healthy individuals. Plasma concentration of BuChE was measured by the chemiluminescent method and AChE H353N polymorphism was investigated by PCR-RFLP and sequencing of the respective encoding AChE gene fragment. The BuChE concentration was statistically higher in Chagas disease patients, with no AChE genotype significant influence. AChE genotypes YT{*}A/YT{*}A, YT{*}A/YT{*}B and YT{*}B/YT{*}B, respectively, were expressed in 53 (86.88%), 7 (11.46%) and one (1.64%) chagasic patients, and in 68 (91.89%), 6 (8.10%) and none healthy individuals. BuChE activity may represent an important marker for chronic Chagas disease inflammatory process and prognostic. Lower BuChE concentration correlated with AChE YT{*}B allele, although without statistical power. (AU)

• Processo FAPESP: 11/23378-3 - Estudo das respostas contráteis do cólon de camundongos infectados com formas tripomastigotas sanguíneos da cepa QM2 de Trypanosoma Cruzi
• Beneficiário: Luiz Gonzaga Francisco de Assis Barros D'Elia Zanella
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/14166-8 - Pesquisa de mutações no gene da acetilcolinesterase (AChE) através da técnica de restrição por tamanho de fragmento em pacientes chagásicos crônicos
• Beneficiário: André Cardoso Campello
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/14514-4 - Caracterização da quitinase das Espécies de Leishmania endêmicas da América do Sul: utilização no diagnóstico em humanos, cães e flebotomíneos
• Beneficiário: Marcia Aparecida Speranca
• Linha de fomento: Auxílio à Pesquisa - Regular