Kinetic and thermodynamic parameters of nisin thermoinactivation

Autor(es)

de Almeida, Gabriel Moretti ^[1] ; Nobrega Mendonca, Carlos Miguel ^[1] ; Converti, Attilio ^[2] ; de Souza Oliveira, Ricardo Pinheiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, Prof Lineu Prestes 580 Bloco 16, BR-05508000 Sao Paulo - Brazil

^[2] Univ Genoa, Dept Civil Chem & Environm Engn, Pole Chem Engn, Via Opera Pia 15, I-16145 Genoa - Italy

• Tipo de documento: Artigo Científico
• Fonte: Journal of Food Engineering; v. 280, SEP 2020.
• Citações Web of Science: 0

Resumo

Understanding the thermal inactivation of biopreservatives in food matrixes is essential for their proper use in the food industry, enabling reduction of heating time and optimization of heating temperature. With this aim in mind, the effect of heat treatment on the activity and thermostability of commercial nisin from Lactococcus lactis was investigated. Residual activity tests performed for 90 min in the 70-86 degrees C temperature range at pH 3.0 demonstrated that bacteriocin thermoinactivation followed first-order kinetics. Low values of the inactivation rate constant (0.0089-0.0892 min(-1)) and high values of the decimal reduction time (25.8 <= D-value <= 258.4 min) indicated excellent nisin thermostability in the lower end of the investigated temperature range, but the thermal resistance constant (z-value) was only 15.4 degrees C, which pointed out poor thermoresistance at the higher end. Thermodynamic parameters estimated for nisin irreversible thermal inactivation were energy, enthalpy and Gibbs free energy of activation of 155.1, 152.2-152.3 and 83.5-86.6 kJ mol(-1,) respectively, and activation entropy of 188.6-192.5 J mol(-1) K-1. If on the one hand these results taken together confirm the thermostability of nisin as a biopreservative in use in the food industry, on the other hand suggest the importance of looking for new more thermostable antimicrobials to use in operations at higher temperatures. (AU)

• Processo FAPESP: 19/19054-0 - Uso de bactérias lácticas para a produção de bebidas lácteas probióticas e bacteriocinas: aplicação nas indústrias alimentícia e farmacêutica
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
• Processo FAPESP: 18/04385-8 - Construção de uma linhagem recombinante produtora de pediocina a partir de resíduo agroindustrial
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular