Three-Dimensional Cell Culture Based on Magnetic Fields to Assemble Low-Grade Ovarian Carcinoma Cell Aggregates Containing Lymphocytes

Autor(es)

de Souza-Araujo, Caroline Natania ^[1] ; Tonetti, Claudia Rodrigues ^[1] ; Cardoso, Marcella Regina ^[1] ; Lucci de Angelo Andrade, Liliana Aparecida ^[1] ; da Silva, Rodrigo Fernandes ^[1] ; Romani Fernandes, Luis Gustavo ^[1] ; Guimaraes, Fernando ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Med, BR-13083887 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Womens Hosp Jose Aristodemo Pinotti, CAISM, BR-13083881 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELLS; v. 9, n. 3 MAR 2020.
• Citações Web of Science: 0

Resumo

There is a limited number of established ovarian cancer cell lines matching the low-grade serous histotype available for research purposes. Three-dimensional (3D) culture systems provide in vitro models with better tissue-like characteristics than two-dimensional (2D) systems. The goal in the study was to characterize the growth of a given low-grade serous ovarian carcinoma cell line in a 3D culture system conducted in a magnetic field. Moreover, the culture system was evaluated in respect to the assembly of malignant cell aggregates containing lymphocytes. CAISMOV24 cell line alone or mixed with human peripheral blood mononuclear cells (PBMC) were cultured using a commercially available 3D culture system designed for 24 well plates. Resulting cell aggregates revealed the intrinsic capacity of CAISMOV24 cells to assemble structures morphologically defined as papillary, and reflected molecular characteristics usually found in ovarian carcinomas. The contents of lymphocytes into co-cultured cell aggregates were significantly higher (p < 0.05) when NanoShuttle-conjugated PBMC were employed compared with non-conjugated PBMC. Moreover, lymphocyte subsets NK, T-CD4, T-CD8 and T-regulatory were successfully retrieved from co-cultured cell aggregates at 72h. Thus, the culture system allowed CAISMOV24 cell line to develop papillary-like cell aggregates containing lymphocytes. (AU)

• Processo FAPESP: 18/06959-1 - Avaliação das vias de sinalização intracelular JAK-STAT5 e mTOR-S6 na modulação funcional das células natural killer (NK) de pacientes com câncer epitelial de ovário
• Beneficiário: Fernando Guimarães
• Modalidade de apoio: Auxílio à Pesquisa - Regular