Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples

Autor(es)

Rocha, Giovanna S. ^[1] ; Silva, Martin K. L. ^[1] ; Cesarino, Ivana ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Agr, Dept Bioproc & Biotechnol, BR-18610034 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS; v. 13, n. 7 APR 2020.
• Citações Web of Science: 4

Resumo

A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from Staphylococcus aureus in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L-1 via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples. (AU)

• Processo FAPESP: 17/07288-0 - Desenvolvimento de imunossensor baseado em grafeno para detecção da enterotoxina A de Staphylococcus aureus em amostras de leite
• Beneficiário: Giovanna da Silva Rocha
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica