Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples

Rocha, Giovanna S.; Silva, Martin K. L.; Cesarino, Ivana

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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples

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Author(s):	Rocha, Giovanna S. ^[1] ; Silva, Martin K. L. ^[1] ; Cesarino, Ivana ^[1] Total Authors: 3
Affiliation:	^[1] Sao Paulo State Univ UNESP, Sch Agr, Dept Bioproc & Biotechnol, BR-18610034 Botucatu, SP - Brazil Total Affiliations: 1
Document type:	Journal article
Source:	MATERIALS; v. 13, n. 7 APR 2020.
Web of Science Citations:	4
Abstract
A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from Staphylococcus aureus in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L-1 via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples. (AU)

FAPESP's process:	17/07288-0 - Development of an immunosensor based on graphene for the detection of enterotoxin A of Staphylococcus aureus in milk samples
Grantee:	Giovanna da Silva Rocha
Support Opportunities:	Scholarships in Brazil - Scientific Initiation

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