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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes

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Autor(es):
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Leticia Gomes de Pontes [1] ; Wanessa Fernanda Altei [2] ; Asier Galan [3] ; Petra Bili [4] ; Nicolas Guillemin [5] ; Josipa Kule [6] ; Anita Horvati [7] ; Lígia Nunes de Morais Ribeiro [8] ; Eneida de Paula [9] ; Virgínia Bodelão Richini Pereira [10] ; Simone Baldini Lucheis [11] ; Vladimir Mrljak [12] ; Peter David Eckersall ; Rui Seabra Ferreira Jr ; Lucilene Delazari dos Santos
Número total de Autores: 15
Afiliação do(s) autor(es):
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[1] São Paulo State University. Botucatu Medical School. Graduate Program in Tropical Diseases - Brasil
[2] Federal University of São Carlos. Department of Physiological Sciences. Laboratory of Biochemistry and Molecular Biology - Brasil
[3] University of Zagreb. Faculty of Veterinary Medicine. Clinic for Internal Diseases
[4] University of Zagreb. Faculty of Veterinary Medicine. Clinic for Internal Diseases
[5] University of Zagreb. Faculty of Veterinary Medicine. Clinic for Internal Diseases
[6] University of Zagreb. Faculty of Veterinary Medicine. Clinic for Internal Diseases
[7] University of Zagreb. Faculty of Veterinary Medicine. Clinic for Internal Diseases
[8] University of Campinas. Institute of Biology. Department of Biochemistry and Tissue Biology - Brasil
[9] University of Campinas. Institute of Biology. Department of Biochemistry and Tissue Biology - Brasil
[10] Adolfo Lutz Institute. Center of Regional Laboratories II - Brasil
[11] Paulista Agency of Agribusiness Technology - Brasil
[12] University of Zagreb. Faculty of Veterinary Medicine. Clinic for Internal Diseases
Número total de Afiliações: 15
Tipo de documento: Artigo Científico
Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 26, 2020-05-29.
Resumo

Abstract Background: Extracellular vesicles (EVs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. The aim of the present report was to provide the first isolation, quantification and protein characterization of EVs from buffalo (Bubalus bubalis) sera infected with Theileria spp. Methods: Infected animals were identified through optical microscopy and PCR. EVs were isolated from buffalo sera by size-exclusion chromatography and characterized using western blotting analysis, nanoparticle tracking analysis and transmission electron microscopy. Subsequently, the proteins from isolated vesicles were characterized by mass spectrometry. Results: EVs from buffalo sera have shown sizes in the 124-140 nm range and 306 proteins were characterized. The protein-protein interaction analysis has evidenced biological processes and molecular function associated with signal transduction, binding, regulation of metabolic processes, transport, catalytic activity and response to acute stress. Five proteins have been shown to be differentially expressed between the control group and that infected with Theileria spp., all acting in the oxidative stress pathway. Conclusions: EVs from buffaloes infected with Theileria spp. were successfully isolated and characterized. This is an advance in the knowledge of host-parasite relationship that contributes to the understanding of host immune response and theileriosis evasion mechanisms. These findings may pave the way for searching new EVs candidate-markers for a better production of safe biological products derived from buffaloes. (AU)

Processo FAPESP: 14/13299-7 - Identificação de biomarcadores moleculares prognósticos e diagnósticos de cicatrização no exsudato inflamatório de úlceras venosas crônicas antes e após o tratamento com selante de fibrina
Beneficiário:Lucilene Delazari dos Santos
Modalidade de apoio: Auxílio à Pesquisa - Regular