Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Vibrational Spectroscopic Characterization and Coherent Anti-Stokes Raman Spectroscopy (CARS) Imaging of Artepillin C

Texto completo
Autor(es):
Pazin, Wallance M. [1, 2] ; Furini, Leonardo N. [3, 1] ; Solovyeva, Vita [2, 4] ; Lemma, Tibebe [1] ; Rubira, Rafael J. G. [1] ; Jorgensen, Bjarke [5] ; Constantino, Carlos J. L. [1] ; Brewer, Jonathan R. [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Sch Technol & Appl Sci, Dept Phys, Presidente Prudente - Brazil
[2] Univ Southern Denmark, Dept Biochem & Mol Biol BMB, Odense - Denmark
[3] Univ Fed Ceara, Dept Phys, Fortaleza, Ceara - Brazil
[4] Carl von Ossietzky Univ Oldenburg, Fac Math & Sci, Oldenburg - Germany
[5] Newtec Engn AS, Odense - Denmark
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Applied Spectroscopy; v. 74, n. 7 APR 2020.
Citações Web of Science: 0
Resumo

In the following work, the vibrational spectroscopic characteristics of artepillin C are reported by means of Fourier transform infrared (FT-IR) and Raman spectroscopies, surface-enhanced Raman scattering (SERS), and coherent anti-Stokes Raman scattering (CARS) microscopy. Artepillin C is an interesting compound due to its pharmacological properties, including antitumor activity. It is found as the major component of Brazilian green propolis, a resinous mixture produced by bees to protect their hives against intruders. Vibrational spectroscopic techniques have shown a strong peak at 1599 cm(-1), assigned to C=C stretching vibrations from the aromatic ring of artepillin C. From these data, direct visualization of artepillin C could be assessed by means of CARS microscopy, showing differences in the film hydration obtained for its neutral and deprotonated states. Raman-based methods show potential to visualize the uptake and action of artepillin C in biological systems, triggering its interaction with biological systems that are needed to understand its mechanism of action. (AU)

Processo FAPESP: 16/09633-4 - Mecanismo de ação do composto bioativo Artepillin C em sistemas miméticos de membranas e membranas citoplasmáticas de células tumorais
Beneficiário:Wallance Moreira Pazin
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 17/23426-4 - Efeitos da interação da Artepillin C com membranas de células tumorais: um estudo por microscopia de fluorescência avançada
Beneficiário:Wallance Moreira Pazin
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado