| Full text | |
| Author(s): |
Pazin, Wallance M.
[1, 2]
;
Furini, Leonardo N.
[3, 1]
;
Solovyeva, Vita
[2, 4]
;
Lemma, Tibebe
[1]
;
Rubira, Rafael J. G.
[1]
;
Jorgensen, Bjarke
[5]
;
Constantino, Carlos J. L.
[1]
;
Brewer, Jonathan R.
[2]
Total Authors: 8
|
| Affiliation: | [1] Sao Paulo State Univ, Sch Technol & Appl Sci, Dept Phys, Presidente Prudente - Brazil
[2] Univ Southern Denmark, Dept Biochem & Mol Biol BMB, Odense - Denmark
[3] Univ Fed Ceara, Dept Phys, Fortaleza, Ceara - Brazil
[4] Carl von Ossietzky Univ Oldenburg, Fac Math & Sci, Oldenburg - Germany
[5] Newtec Engn AS, Odense - Denmark
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Applied Spectroscopy; v. 74, n. 7 APR 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
In the following work, the vibrational spectroscopic characteristics of artepillin C are reported by means of Fourier transform infrared (FT-IR) and Raman spectroscopies, surface-enhanced Raman scattering (SERS), and coherent anti-Stokes Raman scattering (CARS) microscopy. Artepillin C is an interesting compound due to its pharmacological properties, including antitumor activity. It is found as the major component of Brazilian green propolis, a resinous mixture produced by bees to protect their hives against intruders. Vibrational spectroscopic techniques have shown a strong peak at 1599 cm(-1), assigned to C=C stretching vibrations from the aromatic ring of artepillin C. From these data, direct visualization of artepillin C could be assessed by means of CARS microscopy, showing differences in the film hydration obtained for its neutral and deprotonated states. Raman-based methods show potential to visualize the uptake and action of artepillin C in biological systems, triggering its interaction with biological systems that are needed to understand its mechanism of action. (AU) | |
| FAPESP's process: | 16/09633-4 - Mechanism of action of the bioactive compound Artepillin C in membrane biomimetic systems and cytoplasmic membrane of tumor cells |
| Grantee: | Wallance Moreira Pazin |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/23426-4 - Insights on the interaction of Artepillin C with tumor cell membranes: an advanced fluorescence microscopy study |
| Grantee: | Wallance Moreira Pazin |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |