Effect of interfering agents for urease immobilized in Langmuir-Blodgett films of controlled molecular architecture

Autor(es)

Rodrigues, Raul Torres ^[1] ; Souza Nordi, Cristiana Freire ^[1] ; Siqueira Junior, Jose Roberto ^[2] ; Caseli, Luciano ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Inst Environm Chem & Pharmaceut Sci, Diadema, SP - Brazil

^[2] Fed Univ Triangulo Mineiro UFTM, Inst Nat Exact Sci & Educ, Uberaba, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Thin Solid Films; v. 704, JUN 30 2020.
• Citações Web of Science: 0

Resumo

Catalytic performance of enzymes confined in lipid Langmuir-Blodgett (LB) films can be tuned if the molecular architecture and environment are controlled. In this paper, nanohybrid LB films constituted of dioctadecyldimethylammonium bromide, algal polysaccharides, carbon nanotubes and urease had its catalytic activity investigated by adding possible interfering ions in the aqueous environment that contained urea in contact with the LB film. Catalytic activity of LB film towards urea hydrolysis was treated as obeying the Michaelis-Menten mechanism and the effect of ascorbate, oxalate, sulfate, phosphate and their respective counterions were measured. While oxalate and ascorbic acid inactivated totally the catalytic activity, sodium, potassium, sulfate and phosphate ions inhibited only partially. Therefore, these experiments gave us insight about the mechanism of enzymes confined in ultrathin films for biosensing. (AU)

• Processo FAPESP: 19/03239-0 - Interfaces nanoestruturadas para a investigação de substâncias bioativas em modelos de membrana celular e para a construção de dispositivos optoeletrônicos enzimáticos
• Beneficiário: Luciano Caseli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/01867-6 - Filmes Langmuir-Blodgett híbridos de nanotubos de carbono e polissacarídeos algais para imobilização de enzimas
• Beneficiário: Raul Torres Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica