Gram-negative bacteria carrying beta-lactamase encoding genes in hospital and urban wastewater in Brazil

Autor(es)

Zagui, Guilherme Sgobbi ^[1] ; de Andrade, Leonardo Neves ^[2] ; Moreira, Natalia Columbaro ^[2] ; Silva, Thais Vilela ^[1] ; Machado, Gabriel Pinheiro ^[1] ; da Costa Darini, Ana Lucia ^[2] ; Segura-Munoz, Susana Ines ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Slo Paulo, Coll Nursing Ribeirao Preto, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Coll Nursing Ribeirao Preto, Lab Ecotoxicol & Environm Parasitol, Dept Maternal Infant Nursing & Publ Hlth, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL MONITORING AND ASSESSMENT; v. 192, n. 6 MAY 17 2020.
• Citações Web of Science: 0

Resumo

Multidrug resistance mediated by beta-lactamase in Gram-negative bacilli is a serious public health problem. Sewers are considered reservoirs of multiresistant bacteria due to presence of antibiotics that select them and favor their dissemination. The present study evaluated the antibiotic resistance profile and beta-lactamases production in Gram-negative bacilli isolates from hospital sewage and urban wastewater treatment plants (UWWTP) in Brazil. Bacteria were isolated and identified with biochemical tests. Antibiotic susceptibility testing was performed by the disk-diffusion method and detection of extended-spectrum beta-lactamase and carbapenemases by enzymatic inhibitor and conventional PCR. Differences in resistance to amoxicillin clavulanic, aztreonam, cefepime, and cefotaxime were observed in hospital sewage compared with urban sewage (p < 0.05). The multidrug-resistant phenotype was observed in 33.3% of hospital sewage isolates (p = 0.0025). beta-lactamases genes were found in 35.6% of isolates, with the most frequent being bla(KPC) and bla(TEM) (17.8%), and bla(SHV) and bla(CTX-M) (13.3% and 8.9%, respectively). The data obtained are relevant, since the bacteria detected are on the priority pathogens list from the World Health Organization and hospital sewage could be released untreated into the municipal collection system, which may favor the spread of resistance. Changes in hospital sewage discharge practices, as well as additional technologies regarding effluent disinfection in the UWWTP, can prevent the spread of these bacteria into the environment and negative impact on water resources. (AU)

• Processo FAPESP: 17/10723-0 - Avaliação da multirresistência aos antibióticos de bactérias isoladas de efluente hospitalar e domiciliar: determinação da sobrevida ao tratamento de esgoto e de enzimas inativadoras de antibióticos
• Beneficiário: Guilherme Sgobbi Zagui
• Modalidade de apoio: Bolsas no Brasil - Mestrado