H-1-NMR Based Serum Metabolomics Identifies Different Profile between Sarcopenia and Cancer Cachexia in Ageing Walker 256 Tumour-Bearing Rats

Autor(es)

Viana, Lais Rosa ^[1] ; Lopes-Aguiar, Leisa ^[1] ; Rosolen, Rafaela Rossi ^[1] ; dos Santos, Rogerio Willians ^[1] ; Cintra Gomes-Marcondes, Maria Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Biol Inst, Dept Struct & Funct Biol, Lab Nutr & Canc, Campinas 13083862 - Brazil

• Tipo de documento: Artigo Científico
• Fonte: METABOLITES; v. 10, n. 4 APR 2020.
• Citações Web of Science: 0

Resumo

Sarcopenia among the older population has been growing over the last few years. In addition, the incidence of cancers increases with age and, consequently, the development of cachexia related cancer. Therefore, the elucidation of the metabolic derangements of sarcopenia and cachexia are important to improve the survival and life quality of cancer patients. We performed the H-1-NMR based serum metabolomics in adult (A) and ageing (S) Walker 256 tumour-bearing rats in different stages of tumour evolution, namely intermediated (Wi) and advanced (Wa). Among 52 serum metabolites that were identified, 21 were significantly increased in S and 14 and 19 decreased in the Wi and Wa groups, respectively. The most impacted pathways by this metabolic alteration were related by amino acid biosynthesis and metabolism, with an upregulation in S group and downregulation in Wi and Wa groups. Taken together, our results suggest that the increase in metabolic profile in ageing rats is associated with the higher muscle protein degradation that releases several metabolites, especially amino acids into the serum. On the other hand, we hypothesise that the majority of metabolites released by muscle catabolism are used by tumours to sustain rapid cell proliferation and tumorigenesis. (AU)

• Processo FAPESP: 15/21890-0 - Efeito das dietas leucina, cetogênica e combinada no crescimento tumoral, estado caquético e perfil metabolômico sistêmico, muscular e tumoral de ratas portadoras de câncer caquexia (modelo carcinossarcoma de Walker 256)
• Beneficiário: Laís Rosa Viana
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/06955-0 - Avaliação do perfil metabolômico, proteômico, das vias de proliferação celular e apoptose do câncer sob influência dos hormônios da gravidez em ratas submetidas à dieta rica em leucina
• Beneficiário: Laís Rosa Viana
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/02739-4 - Nutrição e câncer: estudo dos aspectos moleculares, proteômicos e metabolômicos em modelo experimental de caquexia
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/13334-7 - Nutrição e câncer: avaliação do perfil metabolômico, proteômico e das vias de sinalização celular muscular, placentária e fetal e do tecido tumoral sob influência dos hormônios da gravidez em ratas
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/20558-2 - Nutrição e câncer. estudo dos aspectos moleculares, proteômicos e metabolômicos em modelo experimental de caquexia
• Beneficiário: Leisa Lopes Aguiar
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado