Healthcare-associated carbapenem-resistant OXA-72-producing Acinetobacter baumannii of the clonal complex CC79 colonizing migratory and captive aquatic birds in a Brazilian Zoo

Autor(es)

Narciso, Ana Clara ^[1] ; Martins, Willames M. B. S. ^[1] ; Almeida, Luiz G. P. ^[2] ; Cayo, Rodrigo ^{[1, 3]} ; Santos, Stefanie Vanessa ^[1] ; Ramos, Patricia Locosque ^[4] ; Lincopan, Nilton ^[5] ; Vasconcelos, Ana Tereza R. ^[2] ; Gales, Ana Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Dept Internal Med, LAB Alerta, Div Infect Dis, Sao Paulo - Brazil

^[2] Lab Nacl Comp Cient LNCC, Lab Bioinformat, Petropolis, RJ - Brazil

^[3] Univ Fed Sao Paulo, Inst Ciancias Ambientais Quim & Farmacaut, Dept Ciencias Biol, Lab Bacteriol & Imunol, Diadema, SP - Brazil

^[4] Fundacao Parque Zool Sao Paulo FPZSP, Dept Pesquisas Aplicadas, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Science of The Total Environment; v. 726, JUL 15 2020.
• Citações Web of Science: 0

Resumo

Carbapenem resistance in Acinetobacter baumannii is a public health issue globally, mainly due to the production of carbapenem hydrolyzing class D beta-lactamases (CHDLs). In Brazil, OXA-23 and OXA-143 CHDLs have been prevalent in A. baumannii from clinical settings, with some OXA-23 reports in the environmental samples, whereas OXA-72 has begun to be increasingly reported. This study aims to perform the genomic and microbiological characterization of carbapenem-resistant A. baumannii isolates recovered from migratory birds and captive birds inhabiting a lake within a Brazilian Zoo. Four hundred and eighty-one gram-negative bacilli were recovered from choanal and cloacal swabs obtained from 50 migratory birds and 37 captive birds present at the zoo's lake between July and August of 2012. Among all GNB, nine OXA-72-producing A. baumannii were detected from the microbiota of four migratory and five captive aquatic birds. The OXA-72-producing A. baumannii isolates were submitted to antimicrobial susceptibility test and PFGE, exhibiting a multidrug-resistant profile and clonal relatedness with OXA-72-positive human isolates circulating for eighteen years in a hospital setting. MLST, plasmid analysis and whole-genome sequencing revealed which all carbapenem-resistant A. baumannii from bird and human hosts belonged to clonal complex 79, and harboured a small plasmid (similar to 16.6-kb in size), named pAC1-BRL, which carried bla(OXA-72) gene, macrolide resistance genes msrE and mphE, and the toxin-antitoxin system AbkAB. To determine the impact of pAC1-BRL acquisition in the the capacity of a microorganism to survive in a competitive environment (in the following called fitness), the laboratory strain A. baumannii ATCC 19606 was used in the fitness experiments and suggested an increase of its relative fitness after the pAC1-BRL acquisition. In summary, the detection of OXA-72-producing A. baumannii strains belonging to CC79 in aquatic birds is a piece of epidemiological evidence demonstrating that dissemination of high-risk bacteria is extending beyond the hospital. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 17/02258-6 - Avaliação do papel da AVE migratória Dendrocygna viduata na disseminação de bacilos gram-negativos resistentes a antimicrobianos
• Beneficiário: Ana Cristina Gales
• Linha de fomento: Auxílio à Pesquisa - Regular