Advances in the Biosynthesis of Pyranocoumarins: Isolation and C-13-Incorporation Analysis by High-Performance Liquid Chromatography-Ultraviolet-Solid-Phase Extraction-Nuclear Magnetic Resonance Data

Autor(es):Mostrar menos -

Amaral, Jessica C. ^[1] ; da Silva, Michelli M. ^[1] ; da Silva, M. Fatima G. F. ^[1] ; Alves, Thayana C. ^[1] ; Ferreira, A. Gilberto ^[1] ; Forim, Moacir R. ^[1] ; Fernandes, Joao B. ^[1] ; Pina, Edieidia S. ^[2] ; Lopes, Adriana A. ^[2] ; Pereira, Ana M. S. ^[2] ; Novelli, Valdenice M. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Ribeirao Preto, Unidade Biotecnol, BR-14096900 Ribeirao Preto, SP - Brazil

^[3] Ctr APTA Citros Sylvio Moreira, Inst Agron, BR-13490970 Cordeiropolis, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Natural Products; v. 83, n. 5, p. 1409-1415, MAY 22 2020.
• Citações Web of Science: 0

Resumo

Citrus sinensis and Citrus limonia were obtained by germination from seeds, and isotopic-labeling experiments using D-{[}1-C-13]glucose were performed with the seedlings. After 60 days, the seedlings were analyzed by high-performance liquid chromatography-ultraviolet-solid-phase extraction-nuclear magnetic resonance, data and the C-13 enrichment patterns of xanthyletin and seselin indicated that the pyran ring was formed by the methylerythritol phosphate pathway and that the coumarin moiety was derived from the shikimate pathway in both compounds. This information regarding the biosynthetic pathway can be used to increase resistance against phytopathogens, because xanthyletin and seselin are reported to have antimicrobial activity on the growth of Xylella fastidiosa, which causes citrus variegated chlorosis in orange. (AU)

• Processo FAPESP: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock
• Beneficiário: Arlene Gonçalves Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia
• Processo FAPESP: 12/25299-6 - Estudos integrados para o controle de formigas cortadeiras
• Beneficiário: João Batista Fernandes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/50918-7 - INCT 2014: para o Controle Biorracional de Insetos Pragas e Fitopatógenos
• Beneficiário: Maria Fátima das Graças Fernandes da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Temático