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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Advances in the Biosynthesis of Pyranocoumarins: Isolation and C-13-Incorporation Analysis by High-Performance Liquid Chromatography-Ultraviolet-Solid-Phase Extraction-Nuclear Magnetic Resonance Data

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Amaral, Jessica C. [1] ; da Silva, Michelli M. [1] ; da Silva, M. Fatima G. F. [1] ; Alves, Thayana C. [1] ; Ferreira, A. Gilberto [1] ; Forim, Moacir R. [1] ; Fernandes, Joao B. [1] ; Pina, Edieidia S. [2] ; Lopes, Adriana A. [2] ; Pereira, Ana M. S. [2] ; Novelli, Valdenice M. [3]
Total Authors: 11
[1] Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Ribeirao Preto, Unidade Biotecnol, BR-14096900 Ribeirao Preto, SP - Brazil
[3] Ctr APTA Citros Sylvio Moreira, Inst Agron, BR-13490970 Cordeiropolis, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Natural Products; v. 83, n. 5, p. 1409-1415, MAY 22 2020.
Web of Science Citations: 0

Citrus sinensis and Citrus limonia were obtained by germination from seeds, and isotopic-labeling experiments using D-{[}1-C-13]glucose were performed with the seedlings. After 60 days, the seedlings were analyzed by high-performance liquid chromatography-ultraviolet-solid-phase extraction-nuclear magnetic resonance, data and the C-13 enrichment patterns of xanthyletin and seselin indicated that the pyran ring was formed by the methylerythritol phosphate pathway and that the coumarin moiety was derived from the shikimate pathway in both compounds. This information regarding the biosynthetic pathway can be used to increase resistance against phytopathogens, because xanthyletin and seselin are reported to have antimicrobial activity on the growth of Xylella fastidiosa, which causes citrus variegated chlorosis in orange. (AU)

FAPESP's process: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock
Grantee:Arlene Gonçalves Corrêa
Support type: Research Grants - Research Centers in Engineering Program
FAPESP's process: 12/25299-6 - Integrated studies for leaf cutting control
Grantee:João Batista Fernandes
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/50918-7 - INCT 2014: for the Biorational Control of Pest-Insect and Phytopathogens
Grantee:Maria Fátima das Graças Fernandes da Silva
Support type: Research Projects - Thematic Grants