Metabolomic studies of Piperaceae species by MS and NMR: development of analytical...
| Grant number: | 10/17935-4 |
| Support type: | Scholarships in Brazil - Doctorate |
| Effective date (Start): | March 01, 2011 |
| Effective date (End): | April 30, 2015 |
| Field of knowledge: | Physical Sciences and Mathematics - Chemistry |
| Principal Investigator: | Ian Castro-Gamboa |
| Grantee: | Alan Cesar Pilon |
| Home Institution: | Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil |
| Associated scholarship(s): | 13/01542-1 - Development of multivariate statistical tools and bioinformatics aiming the metabolomic study of Mendicago truncatula ecotypes using GC and UHPLC-MS, BE.EP.DR |
Abstract The use of drugs from natural sources is as old as humanity itself. However, due to the economic impact caused by globalization, many pharmaceutic industries searched for alternatives to speed-up assays aiming for simpler structural molecules and low-costs budgets. Therefore, the insertion and development of methods of dereplication associated to metabolomics studies through an approach in system biology is a key issue when searching for bioactive molecules. Recently, due to the advancement of technology, the use of hyphenated techniques such as high performance liquid chromatography (HPLC) coupled to spectroscopic methods like nuclear magnetic resonance (NMR) and high resolution mass spectrometry (HRMS) intensified the metabolomic and system biology research in plants and fungi thus, accelerating the selection of bioactive substances. This project aims to study the molecular dynamics in complex matrixes using multivariate analysis and in doing so generating analytical tools to apply as a rational approach towards the study of bioactive natural products and the understanding of their complexity. To accomplish this task, specimens deposited in our bank of extracts, from the Solanaceae family, little studied on their chemical composition but that showed interesting bioactivities form initial biological screenings, will be the object of study on this project. | |