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Development of analytical method by high performance liquid chromatography for quantification of galloylquinic compounds isolated from Copaifera langsdorffii Desf. leaves and its application in seasonal analysis.

Grant number: 12/01336-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): June 01, 2012
Effective date (End): January 31, 2014
Field of knowledge:Health Sciences - Pharmacy - Pharmacognosy
Principal Investigator:Jairo Kenupp Bastos
Grantee:Erick Vicente da Silva Motta
Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Copaifera L. genus belongs to the family Leguminosae Juss., sub-family Caesalpinioideae Kunth. In Brazil, Copaifera species are widely spread in Amazonas, Pará and Ceará states and are worldwide known by the oil-resin that can be sustainable extracted from its trunk, due to extensive use in folk medicine. In particular, C. langsdorffii Desf. species has been studied by several chemist, botanist and pharmacologist researchers. These studies mostly are related to oil-resin or volatile fraction obtained from wood trunk. However, few studies are related to the chemical composition and biological properties of other plant parts, such as leaves, as well as to the development of analytical methodologies to verify the authenticity of these products. So far, only a method to quantify the two major flavonoids has been developed. Since the aerial parts extract is rich in galloylquinic compounds, the aim of this study is to undertake the isolation and identification of quinic acid derivatives, as well as the development of validated analytical method by reversed-phase high-performance liquid chromatography for galloylquinic quantification. For this, different chromatographic techniques will be used for the isolation of the main galloylquinic of the leaves, specialy high-speedy counter-current chromatography. Physical methods, such as nuclear magnetic resonance and mass spectrometry, will be used to identify them. Monthly, qualitative and quantitative analysis of phenolic compounds from 10 population of C. langsdorffii cultivated will also be made in order to study the role of seasonality on the chemical profile of the leaves of this species.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MOTTA, ERICK V. S.; SAMPAIO, BRUNO L.; COSTA, JULIANA C.; TEIXEIRA, SIMONE P.; BASTOS, JAIRO K.. Quantitative analysis of phenolic metabolites in Copaifera langsdorffii leaves from plants of different geographic origins cultivated under the same environmental conditions. Phytochemical Analysis, v. 30, n. 3, p. 364-372, . (11/13630-7, 12/01336-0)
MOTTA, ERICK V. S.; LEMOS, MARIVANE; COSTA, JULIANA C.; BANDERO-FILHO, VILMAR C.; SASSE, ASTRID; SHERIDAN, HELEN; BASTOS, JAIRO K.. Galloylquinic acid derivatives from Copaifera langsdorffii leaves display gastroprotective activity. Chemico-Biological Interactions, v. 261, p. 145-155, . (11/13630-7, 12/09727-8, 12/01336-0)
DA SILVA MOTTA, ERICK VICENTE; DA COSTA, JULIANA DE CARVALHO; BASTOS, JAIRO KENUPP. A validated HPLC-UV method for the analysis of galloylquinic acid derivatives and flavonoids in Copaifera langsdorffii leaves. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1061, p. 240-247, . (12/03852-5, 11/13630-7, 12/01336-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MOTTA, Erick Vicente da Silva. Development and validation of analytical method by HPLC-UV-DAD for quantification and seasonal studies of galloylquinic compounds from Copaifera langsdorffii leaves.. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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