Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women

Autor(es):Mostrar menos -

Vesentini, Giovana ^{[1, 2]} ; Barbosa, Angelica M. P. ^{[2, 3]} ; Damasceno, Debora C. ^[1] ; Marini, Gabriela ^{[1, 2, 4]} ; Piculo, Fernanda ^{[1, 2]} ; Matheus, Selma M. M. ^{[5, 2]} ; Hallur, Raghavendra L. S. ^{[1, 2]} ; Nunes, Sthefanie K. ^{[1, 2]} ; Catinelli, Bruna B. ^{[1, 2]} ; Magalhaes, Claudia G. ^{[1, 2]} ; Costa, Roberto ^{[1, 2]} ; Abbade, Joelcio F. ^{[1, 2]} ; Corrente, Jose E. ^{[2, 6]} ; Calderon, Iracema M. P. ^{[1, 2]} ; Rudge, Marilza V. C. ^{[1, 2]} ; Grp, DIAMATER Study

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Gynecol & Obstet, Botucatu, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Univ Hosp, Perinatal Diabet Res Ctr, Botucatu Med Sch, Botucatu, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Philosophy & Sci, Dept Physiotherapy & Occupat Therapy, Marilia, SP - Brazil

^[4] Univ Sagrad Coracao, Dept Hlth Sci, Bauru, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Inst Biosci, Dept Anat, Botucatu, SP - Brazil

^[6] Sao Paulo State Univ UNESP, Biosci Inst, Dept Biostat, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 15, n. 4 APR 3 2020.
• Citações Web of Science: 0

Resumo

Background and objective In the present study, we compared the effect of diabetic pregnancy on the rectus abdominis muscle (RAM) in humans and rats. We hypothesized that our animal model could provide valuable information about alterations in the RAM of women with Gestational Diabetes (GDM). Method Newborns female rats (n = 10/group) were administered streptozotocin (100 mg/kg body weight) subcutaneously and were mated on reaching adulthood, to develop the mild hyperglycemic pregnant (MHP) rat model. At the end of pregnancy, the mothers were sacrificed, and the RAM tissue was collected. Pregnant women without GDM (non-GDM group; n = 10) and those diagnosed with GDM (GDM group; n = 8) and undergoing treatment were recruited, and RAM samples were obtained at C-section. The RAM architecture and the distribution of the fast and slow fibers and collagen were studied by immunohistochemistry. Results No statistically significant differences in the maternal and fetal characters were observed between the groups in both rats and women. However, significant changes in RAM architecture were observed. Diabetes in pregnancy increased the abundance of slow fibers and decreased fast fiber number and area in both rats and women. A decrease in collagen distribution was observed in GDM women; however, a similar change was not observed in the MHP rats. Conclusion Our results indicated that pregnancy-associated diabetes-induced similar structural adaptations in the RAM of women and rats with slight alterations in fiber type number and area. These findings suggest that the MHP rat model can be used for studying the effects of pregnancy-associated diabetes on the fiber structure of RAM. (AU)

• Processo FAPESP: 16/09710-9 - Caracterização de marcadores do binômio hiperglicemia gestacional e incontinência urinária gestacional na predição de incontinência urinária 6-12 meses pós-parto
• Beneficiário: Marilza Vieira Cunha Rudge
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/01743-5 - Coorte da tríade gestacional: hiperglicemia, incontinência urinária e perfil clínico, molecular e ômico da miopatia hiperglicêmica na predição de incontinência e disfunção muscular e pesquisa translacional com biodevice para regeneração muscular em ratas
• Beneficiário: Marilza Vieira Cunha Rudge
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/26852-6 - Repercussões da hiperglicemia gestacional e incontinência urinária gestacional no músculo reto abdominal: matriz extracelular, expressão proteica e ultraestrutura
• Beneficiário: Giovana Vesentini
• Modalidade de apoio: Bolsas no Brasil - Doutorado