Analysis of Gene Expression of miRNA-106b-5p and TRAIL in the Apoptosis Pathway in Gastric Cancer

Autor(es)

Pereira, Jessica ^[1] ; Santos, Monica ^[1] ; Delabio, Roger ^[1] ; Barbosa, Monica ^[2] ; Smith, Marilia ^[3] ; Payao, Spencer ^[1] ; Rasmussen, Lucas ^[1]

Afiliação do(s) autor(es)

^[1] Marilia Med Sch FAMEMA, BR-17519030 Marilia, SP - Brazil

^[2] Fed Univ Goias UFG, Dept Biosci & Technol, Inst Trop Pathol & Publ Hlth, BR-74605050 Goiania, Go - Brazil

^[3] Fed Univ Sao Paulo UNIFESP, Dept Morphol & Genet, Escola Paulista Med, BR-04023062 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENES; v. 11, n. 4 APR 2020.
• Citações Web of Science: 0

Resumo

Helicobacter pylori (H. pylori) is one of the main causes of gastric gancer. TNF-related apoptosis-inducing ligand (TRAIL) is a protein able to promote apoptosis in cancer cells, however not in gastric cancer, which presents resistance to apoptosis via TRAIL. It is believed that MicroRNA-106b-5p might be involved in this resistance, although its role in Gastric Cancer is unclear. We aimed to determine the expression of microRNA-106b-5p and TRAIL in patients with gastric diseases, infected by H. pylori, and understand the relationship between these genes and their role in apoptosis and the gastric cancer pathways. H. pylori was detected by PCR, gene expression analysis was performed by real-time-qPCR, and bioinformatics analysis was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cytoscape software. A total of 244 patients were divided into groups (Control, Gastritis, and Cancer); H. pylori was detected in 42.2% of the samples. The cancer group had a poor expression of TRAIL (p < 0.0001) and overexpression of microRNA-106b-5p (p = 0.0005), however, our results confirmed that these genes are not directly related to each other although both are apoptosis-related regulators. Our results also indicated that H. pylori decreases microRNA-106b-5p expression and that this is a carcinogenic bacterium responsible for gastric diseases. (AU)

• Processo FAPESP: 18/08481-1 - Análise da Interação Gênica e Proteica entre Componentes das Vias Inflamatória, da Apoptose e MicroRNAs em Doença Dispéptica e Câncer Gástrico
• Beneficiário: Lucas Trevizani Rasmussen
• Modalidade de apoio: Auxílio à Pesquisa - Regular